Anti-Integrin alpha 2+beta 1 抗体 [16B4] (ab30483)

製品の概要

  • 製品名
    Anti-Integrin alpha 2+beta 1 antibody [16B4]
    Integrin alpha 2+beta 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [16B4] to Integrin alpha 2+beta 1
  • 特異性
    We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション
    適用あり: ICC/IF, IP, Flow Cyt, ELISA, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.

  • ポジティブ・コントロール
    • In IF, this antibody gave a positive signal in HepG2 cells.
  • 特記事項

    This antibody clone is manufactured by Abcam.


    ab30483 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled Human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IP Use a concentration of 25 - 50 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 30 - 40 µg/ml.

ターゲット情報

  • 細胞内局在
    Cell Membrane; single-pass type I membrane protein
  • 参照データベース
  • 別名
    • CD29 antibody
    • CD49B antibody
    • Collagen receptor antibody
    • GPIa antibody
    • GPIIA antibody
    • Integrin alpha 2 antibody
    • Integrin beta 1 antibody
    • ITGA2 antibody
    • ITGB1 antibody
    • Platelet antigen Br antibody
    • Platelet glycoprotein GPIa antibody
    • Platelet glycoprotein Ia antibody
    • Platelet membrane glycoprotein Ia antibody
    • Very late activation protein 2 receptor alpha 2 subunit antibody
    • VLA 2 alpha chain antibody
    • VLA 2 antibody
    • VLA4 antibody
    • VLAA2 antibody
    see all

Anti-Integrin alpha 2+beta 1 antibody [16B4] 画像

  • Overlay histogram showing HT1080 cells stained with ab30483 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab30483, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HT1080 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ICC/IF image of ab30483 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30483, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.
  • ab30483 staining human breast cancer cells by ICC/IF.  Cells were PFA fixed and blocked with 1% serum for 16 hours at 20°C prior to incubating with ab30483 (at 30µg/ml) for 16 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/200, was used as the secondary.

    See Abreview

Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483) 使用論文

This product has been referenced in:
  • Chung CH  et al. Aggretin Venom Polypeptide as a Novel Anti-angiogenesis Agent by Targeting Integrin alpha2beta1. Sci Rep 7:43612 (2017). Read more (PubMed: 28252668) »
  • Suarez E  et al. Identification of biomarkers involved in differential profiling of hypertrophic and keloid scars versus normal skin. Arch Dermatol Res 307:115-33 (2015). Read more (PubMed: 25322916) »

See all 8 Publications for this product

Product Wall

Thank you for your reply.


Based on what you have already tested, I am not sure there are many more steps to be optimized. One consideration would be to extend the primary antibody incubation to overnight at 4oC. Longer incubation with l...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate Cancer cells (PC3))
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Treatment
Anticancer drug
Specification
Prostate Cancer cells (PC3)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 09 2016

Thank you once again for your telephone enquiry.

I am sorry to confirm that this antibody has been tested externally in ELISA and regrettably we have no further details to provide on the protocol on this occasion.

We aim to provid...

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Thank you for your enquiry.

I am sorry I am not able to confirm much further detail regarding the immunogen for ab30483 Anti-Integrin alpha 2+beta 1 antibodyas this particular product has been sourced externally.

However, I can co...

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Thank you for getting back to me with an update of your progress.

I have gone back and had a look at the previous results of your colleague. There definitely seems to be some staining of fibroblasts (in the boiled sample). As you say, it may ...

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Thank you for providing that information. It has helped greatly in understanding what you have been observing.


To answer your question about what staining you can expect with anti-Integrin alpha 2+beta 1 antibody (ab30483) in human skin...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast Cancer Cells)
Specification
Breast Cancer Cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Dr. Sharon Sneddon

Verified customer

投稿 Jul 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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