Anti-Integrin alpha 2b + beta 3 抗体 [237] (ab662)

製品の概要

  • 製品名
    Anti-Integrin alpha 2b + beta 3 antibody [237]
    Integrin alpha 2b + beta 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [237] to Integrin alpha 2b + beta 3
  • 特異性
    Under reducing conditions, this antibody will bind against the human platelet membrane glycoproteins IIb and/or IIIa, which have molecular weights of ~130,000 (IIb) and 115,000 (IIIa).
  • アプリケーション
    適用あり: IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    109 human platelet suspension. The supernatant fluid from postitive clones was screened via ELISA, the solid phase being coated with washed platelets.

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

This antibody, used at a concentration of 10-20µg/mL will allow visualization of NP 40 solubized normal human platelets

ターゲット情報

画像

  • Overlay histogram showing HL60 cells stained with ab662 (red line). The cells were fixed with 4% formaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab662, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

参考文献

This product has been referenced in:
  • Li X  et al. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells. Sci Rep 7:5081 (2017). WB . Read more (PubMed: 28698547) »
  • Lissat A  et al. IL6 secreted by Ewing sarcoma tumor microenvironment confers anti-apoptotic and cell-disseminating paracrine responses in Ewing sarcoma cells. BMC Cancer 15:552 (2015). IHC . Read more (PubMed: 26215971) »

See all 6 Publications for this product

レビューと Q&A

Thank you for contacting us.

The antibody ab662 will detect both alpha 2b and beta 3. I am not sure why this antibody is chosen.

There are other antibodies in catalogue e.g. ab63983, ab134131, ab83961 or ab96794

I hope this i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - other (Breast cancer cells)
Total protein in input
200 µg
Specification
Breast cancer cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

投稿 Apr 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (megakaryocytic)
Loading amount
2e+006 cells
Specification
megakaryocytic
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Dec 13 2006

Ab662 is Protein G Affinity Purified. The product has not been tested for cross-reactivity with other glycoproteins, nor is it known whether it has a greater binding affinity for IIb over IIIa or vice-versa. Ab662 has been used under reducing conditio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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