Anti-Integrin alpha 2 抗体 [HAS-3] (ab10800)

Abreviews (4)Q&A (9)Specific References (5)

製品の概要

  • 製品名
    Anti-Integrin alpha 2 antibody [HAS-3]
    Integrin alpha 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HAS-3] to Integrin alpha 2
  • 由来種
    Mouse
  • アプリケーション
    適用あり: ICC/IF, IP, Flow Cyt, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Whole human keratinocytes.

  • 特記事項


    The antibody co-precipitates alpha 2 beta 1 integrin from keratinocytes and inhibits HGF induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20435685
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
  • 配列類似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメイン
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • BR antibody
      • CD 49b antibody
      • CD49 antigen like family member B antibody
      • CD49 antigen-like family member B antibody
      • CD49b antibody
      • CD49b antigen antibody
      • Collagen receptor antibody
      • DX5 antibody
      • Glycoprotein Ia deficiency included antibody
      • GP Ia antibody
      • GP Ia deficiency, included antibody
      • GPIa antibody
      • HPA 5 included antibody
      • HPA5 included antibody
      • Human platelet alloantigen system 5 antibody
      • Integrin alpha 2 antibody
      • Integrin alpha II antibody
      • Integrin alpha-2 antibody
      • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
      • ITA2_HUMAN antibody
      • ITGA2 antibody
      • Platelet alloantigen Br(a), included antibody
      • Platelet antigen Br antibody
      • Platelet glycoprotein GPIa antibody
      • Platelet glycoprotein Ia antibody
      • Platelet glycoprotein Ia/IIa antibody
      • Platelet membrane glycoprotein Ia antibody
      • Platelet receptor for collagen, deficiency of, included antibody
      • Very late activation protein 2 receptor alpha 2 subunit antibody
      • VLA 2 alpha chain antibody
      • VLA 2 antibody
      • VLA 2 subunit alpha antibody
      • VLA-2 subunit alpha antibody
      • VLA2 antibody
      • VLA2 receptor alpha 2 subunit antibody
      • VLAA2 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [HAS-3] (ab10800)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [HAS-3] (ab10800)This image is courtesy of an anonymous Abreview
      Immunohistochemical analysis of Human blood vessels, staining Integrin alpha 2 with ab10800.

      Tissue was fixed with formaldehyde and blocked with 3% goat serum for 30 minutes at 21°C; antigen retrieval was by an enzymatic method. Samples were incubated with primary antibody (1/10 in diluent) for 1 hour at 21°C. An undiluted streptavidin-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 2 antibody [HAS-3] (ab10800)
      Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 2 antibody [HAS-3] (ab10800)Image from van Leenders GJ et al., PLoS One. 2011;6(11):e26753. Epub 2011 Nov 14. Fig 9.; doi:10.1371/journal.pone.0026753; November 14, 2011, PLoS ONE 6(11): e26753.
      Immunohistochemical analysis of Human prostate cancer tissue, staining Integrin alpha 2 with ab10800. Samples were incubated with primary antibody (1/100) overnight at 4°C. An AlexaFLuor®488-conjugated anti-mouse IgG (1/100) was used as the secondary antibody.

    参考文献

    This product has been referenced in:
    • van Leenders GJ  et al. Activation of c-MET Induces a Stem-Like Phenotype in Human Prostate Cancer. PLoS One 6:e26753 (2011). IHC-Fr ; Human . Read more (PubMed: 22110593) »
    • Singh SR  et al. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol 299:L169-83 (2010). ICC/IF ; Human . Read more (PubMed: 20435685) »

    See all 5 Publications for this product

    ab10800 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    13 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 2 antibody [HAS-3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Vessels)
    Specification
    Vessels
    Fixative
    Formaldehyde
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Pronase K
    Permeabilization
    No
    Blocking step
    Goat-Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Nov 06 2012

    I want to take part.



    Thank you very much for your offer!



    Best regards,

    DISCOUNT CODE: ABTRIAL-000000

    Expiration date: 01. March 2013

    Value: €370.00

    I am very pleased to hear you would like to use ab10800 in IHC and send us an image of your results.

    This code will give you a &eu...

    Read More


    I'll call up to order today.
    Thank you!

    I'm happy to help! Please let me know if you need anything further.


    With the abreview that I submitted for ab10800 published, how do I go
    about claiming for the free antibody? I am interested in purchasing
    the rabbit polyclonal anti-EpCAM antibody (ab71916).
    Thank you.

    Thanks for your enquiry.

    My name is Jeremy Kasanov and I am filling in for Sybille while she is out of the office.

    If you have submitted your review with the discount code in the 'Additional notes' section, you have doone all that i...

    Read More

    ReviewID: xxx AbID: 10800, Anti-Integrin alpha 2 antibody [HAS-3] Rating: Poor Image: Anti-Integrin alpha 2 antibody [HAS-3] for Western blot (Rat) Sample: Species: Rat Type: Cell lysate - whole cell Loading amount: 20 µg Specification: Uterine e...

    Read More

    Thank you for submitting an Abreview for ab10800. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in an untested species and application, we would like to follow ...

    Read More

    Western blot abreview for Anti-Integrin alpha 2 antibody [HAS-3]

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Uterine epithelial cells)
    Loading amount
    20 µg
    Specification
    Uterine epithelial cells
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (10% acrylamide)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jul 25 2012

    Hi,
    Our customer is happy to go ahead and order, they have not purchased the product yet. Her details are as below:
    Name: xxxxxx
    Let me know if you require any further information.
    Kind regards,

    Thank you for confirming that information.
    I have generated the testing discount code as discussed:
    Discount code: xxxxx Expiration date: 29th of September 2012
    Once the Abreview detailing the results of the customers experiment has been...

    Read More

    Dear xxxx,

    Please see customers response:

    I think I will go for the original antibody - ab10800.

    So to be clear, since abcam are offering the testing discount, would this mean that regardless of whether this antibody wo...

    Read More

    Thank you for getting back to me with that confirmation.
    In answer to your question, yes that is how the testing discount works, regardless of if the results with the antibody are positive or negative and the Abreview is submitted with the details...

    Read More

    Dear Supplier,

    I have a customer who is interested in getting a testing discount. Please see customers inquiry below:

    I would like to test the anti-integrin alpha 2 antibody (HAS-3), (ab10800) for the western blot technique in the...

    Read More

    Thank you for contacting us.
    The anti-integrin alpha 2 antibody (HAS-3), (ab10800) your customer is interested in has been raised against whole human keratinocytes. The human integrin alpha 2 protein shares 82% homology with the rat protein. It is...

    Read More

    Dear technical team, Our customer would like to purchase 11 antibodies from abcam and needs control for each of antibody. The customer will test IHC-P so, control should be slide type. I have searched only one of them as below and would like to ask che...

    Read More

    Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the ...

    Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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