Anti-Integrin alpha 2 抗体 [HAS-3] (ab10800)


  • 製品名Anti-Integrin alpha 2 antibody [HAS-3]
    Integrin alpha 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HAS-3] to Integrin alpha 2
  • アプリケーション適用あり: ICC/IF, IP, Flow Cyt, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Whole human keratinocytes.

  • 特記事項

    The antibody co-precipitates alpha 2 beta 1 integrin from keratinocytes and inhibits HGF induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.



Our Abpromise guarantee covers the use of ab10800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20435685
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.


  • 機能Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BR antibody
    • CD 49b antibody
    • CD49 antigen like family member B antibody
    • CD49 antigen-like family member B antibody
    • CD49b antibody
    • CD49b antigen antibody
    • Collagen receptor antibody
    • DX5 antibody
    • Glycoprotein Ia deficiency included antibody
    • GP Ia antibody
    • GP Ia deficiency, included antibody
    • GPIa antibody
    • HPA 5 included antibody
    • HPA5 included antibody
    • Human platelet alloantigen system 5 antibody
    • Integrin alpha 2 antibody
    • Integrin alpha-2 antibody
    • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
    • ITA2_HUMAN antibody
    • ITGA2 antibody
    • Platelet alloantigen Br(a), included antibody
    • Platelet antigen Br antibody
    • Platelet glycoprotein GPIa antibody
    • Platelet glycoprotein Ia antibody
    • Platelet glycoprotein Ia/IIa antibody
    • Platelet membrane glycoprotein Ia antibody
    • Platelet receptor for collagen, deficiency of, included antibody
    • Very late activation protein 2 receptor alpha 2 subunit antibody
    • VLA 2 alpha chain antibody
    • VLA 2 antibody
    • VLA 2 subunit alpha antibody
    • VLA-2 subunit alpha antibody
    • VLA2 antibody
    • VLA2 receptor alpha 2 subunit antibody
    • VLAA2 antibody
    see all

Anti-Integrin alpha 2 antibody [HAS-3] 画像

  • Immunohistochemical analysis of Human blood vessels, staining Integrin alpha 2 with ab10800.

    Tissue was fixed with formaldehyde and blocked with 3% goat serum for 30 minutes at 21°C; antigen retrieval was by an enzymatic method. Samples were incubated with primary antibody (1/10 in diluent) for 1 hour at 21°C. An undiluted streptavidin-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of Human prostate cancer tissue, staining Integrin alpha 2 with ab10800. Samples were incubated with primary antibody (1/100) overnight at 4°C. An AlexaFLuor®488-conjugated anti-mouse IgG (1/100) was used as the secondary antibody.

Anti-Integrin alpha 2 antibody [HAS-3] (ab10800) 使用論文

This product has been referenced in:
  • van Leenders GJ  et al. Activation of c-MET Induces a Stem-Like Phenotype in Human Prostate Cancer. PLoS One 6:e26753 (2011). IHC-Fr ; Human . Read more (PubMed: 22110593) »
  • Singh SR  et al. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol 299:L169-83 (2010). ICC/IF ; Human . Read more (PubMed: 20435685) »

See all 5 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Vessels)
Specification Vessels
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Pronase K
Permeabilization No
Blocking step Goat-Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C

Abcam user community

Verified customer

投稿 Nov 06 2012


Expiration date: 01. March 2013

Value: €370.00

I am very pleased to hear you would like to use ab10800 in IHC and send us an image of your results.

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My name is Jeremy Kasanov and I am filling in for Sybille while she is out of the office.

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Thank you for submitting an Abreview for ab10800. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species and application, we would like to follow ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Uterine epithelial cells)
Loading amount 20 µg
Specification Uterine epithelial cells
Gel Running Conditions Reduced Non-Denaturing (Native) (10% acrylamide)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Jul 25 2012

Thank you for confirming that information.
I have generated the testing discount code as discussed:
Discount code: xxxxx Expiration date: 29th of September 2012
Once the Abreview detailing the results of the customers experiment has been...

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In answer to your question, yes that is how the testing discount works, regardless of if the results with the antibody are positive or negative and the Abreview is submitted with the details...

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Thank you for contacting us.
The anti-integrin alpha 2 antibody (HAS-3), (ab10800) your customer is interested in has been raised against whole human keratinocytes. The human integrin alpha 2 protein shares 82% homology with the rat protein. It is...

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Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the ...

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