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RabMAb

Anti-Integrin alpha 2 抗体 [EPR5789] (ab109432)

製品の概要

  • 製品名
    Anti-Integrin alpha 2 antibody [EPR5789]
    Integrin alpha 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5789] to Integrin alpha 2
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human Integrin alpha 2.

  • ポジティブ・コントロール
    • 293T, T47-D, and A431 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 129 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能
      Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
    • 配列類似性
      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • ドメイン
      The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BR antibody
      • CD 49b antibody
      • CD49 antigen like family member B antibody
      • CD49 antigen-like family member B antibody
      • CD49b antibody
      • CD49b antigen antibody
      • Collagen receptor antibody
      • DX5 antibody
      • Glycoprotein Ia deficiency included antibody
      • GP Ia antibody
      • GP Ia deficiency, included antibody
      • GPIa antibody
      • HPA 5 included antibody
      • HPA5 included antibody
      • Human platelet alloantigen system 5 antibody
      • Integrin alpha 2 antibody
      • Integrin alpha II antibody
      • Integrin alpha-2 antibody
      • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
      • ITA2_HUMAN antibody
      • ITGA2 antibody
      • Platelet alloantigen Br(a), included antibody
      • Platelet antigen Br antibody
      • Platelet glycoprotein GPIa antibody
      • Platelet glycoprotein Ia antibody
      • Platelet glycoprotein Ia/IIa antibody
      • Platelet membrane glycoprotein Ia antibody
      • Platelet receptor for collagen, deficiency of, included antibody
      • Very late activation protein 2 receptor alpha 2 subunit antibody
      • VLA 2 alpha chain antibody
      • VLA 2 antibody
      • VLA 2 subunit alpha antibody
      • VLA-2 subunit alpha antibody
      • VLA2 antibody
      • VLA2 receptor alpha 2 subunit antibody
      • VLAA2 antibody
      see all

    画像



    • Predicted band size : 129 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Integrin alpha 2 knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: T47D cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109432 observed at 165 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109432 was shown to specifically react with Integrin alpha 2 when Integrin alpha 2 knockout samples were used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : T47-D cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 129 kDa

    プロトコール

    参考文献

    This product has been referenced in:
    • Guo F  et al. Gingival fibroblasts display reduced adhesion and spreading on extracellular matrix: a possible basis for scarless tissue repair? PLoS One 6:e27097 (2011). WB ; Human . Read more (PubMed: 22073262) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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