Anti-Integrin alpha 2 抗体 [DX5] (Allophycocyanin) (ab41460)
製品の概要
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製品名Anti-Integrin alpha 2 antibody [DX5] (Allophycocyanin)
Integrin alpha 2 一次抗体 製品一覧 -
製品の詳細Rat monoclonal [DX5] to Integrin alpha 2 (Allophycocyanin)
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由来種Rat
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標識Allophycocyanin. Ex: 645nm, Em: 660nm
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特異性Mouse Integrin alpha 2 (CD49b), 160kDa
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アプリケーション適用あり: Flow Cytmore details
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種交差性交差種: Mouse
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免疫原
RIL-2-propagated NK1.1+ cells from C57BL/6 mice
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特記事項
This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 16% Sucrose, PBS, and stabilizing agent, pH 8.0 -
Concentration information loading... -
精製度Purified IgM
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一次抗体 備考This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.
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ポリ/モノモノクローナル
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クローン名DX5
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ミエローマSp2/0
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アイソタイプIgM
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軽鎖の種類kappa
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab41460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. |
ターゲット情報
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機能Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
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配列類似性Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
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細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 16398 Mouse
- SwissProt: Q62469 Mouse
- Unigene: 5007 Mouse
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別名
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
プロトコール
参考文献
This product has been referenced in:
- Arase H et al. Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2). J Immunol 167:1141-4 (2001). Read more (PubMed: 11466327) »
- Moore TA et al. Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice. J Immunol 157:2366-73 (1996). Read more (PubMed: 8805634) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"