Anti-Integrin alpha 2 抗体 [DX5] (Allophycocyanin) (ab41460)

製品の概要

• 製品名
  Anti-Integrin alpha 2 antibody [DX5] (Allophycocyanin)
  Integrin alpha 2 一次抗体 製品一覧
• 製品の詳細
  Rat monoclonal [DX5] to Integrin alpha 2 (Allophycocyanin)
• 標識
  Allophycocyanin. Ex: 645nm, Em: 660nm
• 特異性
  Mouse Integrin alpha 2 (CD49b), 160kDa
• アプリケーション
  適用あり: Flow Cytmore details
• 種交差性
  交差種: Mouse
  
• 免疫原

  RIL-2-propagated NK1.1+ cells from C57BL/6 mice

• 特記事項

  
  

  This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Store at +4°C.
• バッファー
  Preservative: 0.09% Sodium Azide
  Constituents: 16% Sucrose, PBS, and stabilizing agent, pH 8.0
• Concentration information loading...
• 精製度
  Purified IgM
• 一次抗体 備考
  This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.
• ポリ/モノ
  モノクローナル
• クローン名
  DX5
• ミエローマ
  Sp2/0
• アイソタイプ
  IgM
• 軽鎖の種類
  kappa
• 研究分野
  • Immunology
  • Adaptive Immunity
  • T Cells
  • CD

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Integrins
  • Alpha

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Types of disease
  • Cancer

関連製品

• Compatible Secondaries
  • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
  • Goat Anti-Rat IgG H&L (HRP) (ab205720)

アプリケーション

ターゲット情報

• 機能
  Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
• 配列類似性
  Belongs to the integrin alpha chain family.
  Contains 7 FG-GAP repeats.
  Contains 1 VWFA domain.
• ドメイン
  The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
• 細胞内局在
  Membrane.

• Target information above from: UniProt accession P17301 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 16398 Mouse
  • SwissProt: Q62469 Mouse
  • Unigene: 5007 Mouse
• 別名
  • BR antibody
  • CD 49b antibody
  • CD49 antigen like family member B antibody

  • CD49 antigen-like family member B antibody
  • CD49b antibody
  • CD49b antigen antibody
  • Collagen receptor antibody
  • DX5 antibody
  • Glycoprotein Ia deficiency included antibody
  • GP Ia antibody
  • GP Ia deficiency, included antibody
  • GPIa antibody
  • HPA 5 included antibody
  • HPA5 included antibody
  • Human platelet alloantigen system 5 antibody
  • Integrin alpha 2 antibody
  • Integrin alpha-2 antibody
  • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
  • ITA2_HUMAN antibody
  • ITGA2 antibody
  • Platelet alloantigen Br(a), included antibody
  • Platelet antigen Br antibody
  • Platelet glycoprotein GPIa antibody
  • Platelet glycoprotein Ia antibody
  • Platelet glycoprotein Ia/IIa antibody
  • Platelet membrane glycoprotein Ia antibody
  • Platelet receptor for collagen, deficiency of, included antibody
  • Very late activation protein 2 receptor alpha 2 subunit antibody
  • VLA 2 alpha chain antibody
  • VLA 2 antibody
  • VLA 2 subunit alpha antibody
  • VLA-2 subunit alpha antibody
  • VLA2 antibody
  • VLA2 receptor alpha 2 subunit antibody
  • VLAA2 antibody

  see all

プロトコール

• Flow cytometry protocols