Rabbit polyclonal to Integrin alpha 1
ab78479 is specific to Integrin alpha 1. No cross reactivity to alpha2, alpha3, alpha4, alpha5, alpha6, or alphaV integrin subunits.
Rat, Sheep, Goat, Horse, Chicken, Hamster, Human, Pig, Monkey
Synthetic peptide corresponding to Rat Integrin alpha 1 aa 1166-1180 (C terminal). Cytoplasmic domain.
PC12 cells. Rhesus monkey maternal uterine endometrium tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: Whole serum
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 10
1/100 - 1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 131 kDa).
Use at an assay dependent dilution.
1/200. Suggested for use on acetone fixed tissue only. This antibody has been reported to react in 4% PFA fixed monkey tissue treated with citrate.
Use at an assay dependent concentration.
Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Information by UniProt
CD 49a antibody
CD49 antigen-like family member A antibody
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 1 antibody - Cytoplasmic domain (ab78479)
ICC/IF image of ab78479 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78479, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"