Anti-Insulin Receptor alpha 抗体 (ab5500)

製品の概要

  • 製品名
    Anti-Insulin Receptor alpha antibody
    Insulin Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Insulin Receptor alpha
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide. This information is considered to be commercially sensitive.
    (Peptide available as ab197200)

  • ポジティブ・コントロール
    • WB: SKBR-3 cell lysate and rat and mouse liver tissue lysates. IHC-P: Human breast carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • 特記事項(精製)
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 一次抗体 備考
    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 156 kDa).
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism. Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins.
  • 細胞内局在
    Membrane; single pass type I membrane protein.
  • 参照データベース
  • 別名
    • CD220 antibody
    • HHF5 antibody
    • HIR A antibody
    • INSR antibody
    • Insulin receptor antibody
    • Insulin receptor subunit alpha antibody
    • IR antibody
    see all

画像

  • Anti-Insulin Receptor alpha antibody (ab5500) at 1/1000 dilution + SKBR-3 cell lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 156 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Insulin Receptor alpha using ab5500. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • ab5500 staining Insulin Receptor alpha in Human WBCs by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 2% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in PBS + 2% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (ab150077) (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-Insulin Receptor alpha antibody (ab5500) at 1/2000 dilution + Mouse liver tissue lysate at 20 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 156 kDa

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Insulin Receptor alpha antibody (ab5500) at 1/2000 dilution + Rat liver tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 156 kDa

    Blocking and dilution buffer: 5% NFDM/TBST.

参考文献

This product has been referenced in:
  • Li L  et al. Absence of renal enlargement in fructose-fed proximal-tubule-select insulin receptor (IR), insulin-like-growth factor receptor (IGF1R) double knockout mice. Physiol Rep 4:N/A (2016). Mouse . Read more (PubMed: 27923977) »
  • Martin B  et al. GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging. Front Endocrinol (Lausanne) 6:191 (2015). Read more (PubMed: 26834700) »

See all 4 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (pancreas)
Antigen retrieval step
None - Buffer/Enzyme Used: HistoVTONE(nakaitesque)
Permeabilization
Yes - tween20
Specification
pancreas
Blocking step
Blocking One (nakalaitesque cat:03953-6) as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 25°C
Fixative
Formaldehyde
Username

takai shingo

Verified customer

投稿 Jul 18 2017

Application
Western blot
Sample
Rat Tissue lysate - whole (Kidney tissue)
Gel Running Conditions
Reduced Denaturing (4-20% gel (Bio-Rad))
Loading amount
25 µg
Specification
Kidney tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Sep 15 2015

Thank you for contacting us.

The immunogen sequence homology of this antibody with IGF-1 receptor is 61% so I don't think the antibody would cross react with IGF1 receptor.

I hope this information is helpful to you. Please do not hesi...

Read More

Thank you for this information. We have not tested this product in rat, atlhough it is predicted to work based on homology with the immunogen it is not guaranteed. You may improve the result by increasing your primary antibody dilution to 1/10-1/50 and...

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow)
Specification
Bone marrow
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (WBCs)
Specification
WBCs
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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