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Full length protein (Human).
Our Abpromise guarantee covers the use of ab20756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Sandwich ELISA||Use at an assay dependent concentration.
Can be paired for sandwich ELISA with Mouse monoclonal [D6C4] to Insulin + Proinsulin (ab8304).
|ELISA||Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [D6C4] to Insulin + Proinsulin (ab8304).|
ab20756 staining Insulin + Proinsulin in human pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 and blocked for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) for 1 hour at 25°C followed by incubation with Cy3®-conjugated streptavidin for 30 minutes in the dark at room temperature. Nuclei were counterstained with DAPI.