Anti-Insulin degrading enzyme / IDE 抗体 (ab63945)

製品の概要

  • 製品名Anti-Insulin degrading enzyme / IDE antibody
    Insulin degrading enzyme / IDE 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Insulin degrading enzyme / IDE
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide:

    QQYNFDRDNTE

    , corresponding to C terminal amino acids 914-924 of Human Insulin degrading enzyme/ IDE

  • ポジティブ・コントロール
    • K562 lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
ELISA 1/32000.

ターゲット情報

  • 機能Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
  • 配列類似性Belongs to the peptidase M16 family.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Abeta-degrading protease antibody
    • FLJ35968 antibody
    • Ide antibody
    • IDE_HUMAN antibody
    • Insulin protease antibody
    • Insulin-degrading enzyme antibody
    • Insulinase antibody
    • Insulysin antibody
    • OTTHUMP00000020097 antibody
    see all

Anti-Insulin degrading enzyme / IDE antibody 画像

  • Anti-Insulin degrading enzyme / IDE antibody (ab63945) at 0.1 µg/ml (1 hour) + K562 cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 118 kDa
    Observed band size : 118 kDa

Anti-Insulin degrading enzyme / IDE antibody (ab63945) 使用論文

ab63945 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (mouse embryonic fibroblasts transfected with human)
Loading amount 20 µg
Specification mouse embryonic fibroblasts transfected with human
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Gillian Hunter

Verified customer

投稿 Jul 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"