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Our Abpromise guarantee covers the use of ab6995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. PubMed: 19572802|
|Dot blot||Use at an assay dependent concentration.|
|IHC-P||1/1000. We suggest an incubation period of 30 minutes at room temperature.|
|RIA||Use at an assay dependent concentration.|
ab6995 staining Insulin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 45 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour at 37°C. An Alexa Fluor® 594-conjugated Goat anti-mouse IgG polyclonal (1/400) (ab150116) was used as the secondary antibody.
ab6995 at 1/1000 staining rat pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with BSA before incubation with the antibody for 16 hours at 4°C. An HRP conjugated goat anti-mouse antibody was used as the secondary.