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Preserved by filter sterilization
Our Abpromise guarantee covers the use of ab46707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. Blocks the binding of insulin to the receptor.|
IHC image of Insulin staining in human pancreas formalin fixed paraffin embedded tissue section (ab4611), performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46707, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab46707 staining Insulin in human beta cells grown in vitro in extracellular matrix by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using Triton X-100, then blocked with 2% serum for 2 hours at 25°C. Samples were then incubated with ab46707 at a 1/100 dilution for 20 minutes at 4°C. The secondary used was a Cy3 conjugated goat anti-mouse IgG (ab97035), used at a 1/100 dilution. The nuclei were stained with DAPI.
ab46707 has not yet been referenced specifically in any publications.