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Full length native protein (purified) (Pig).
Our Abpromise guarantee covers the use of ab7760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|RIA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. Blocks the binding of insulin to the receptor. NB: Azide removal is necessary prior to the use of the antibody.|
Ab7760 staining human normal pancreas tissue (ab29816). Staining is localized to extracellular space.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.