Anti-Insulin 抗体 [8E2] (HRP) (ab24455)


  • 製品名Anti-Insulin antibody [8E2] (HRP)
    Insulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8E2] to Insulin (HRP)
  • 標識HRP
  • 特異性Kass for this antibody is 6.0 x 108M-1. It cross-reacts with human proinsulin. No cross-reaction with free C-peptide.
  • アプリケーション適用あり: Sandwich ELISA, ELISAmore details
  • 種交差性
    交差種: Human, Pig
  • 免疫原

    Full length native protein (purified) (Human)




Our Abpromise guarantee covers the use of ab24455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [3A6] to Insulin (ab1965). Can be used as Detection antibody with recommended pair.
ELISA Use at an assay dependent dilution.


  • 機能Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 関連疾患Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 配列類似性Belongs to the insulin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Anti-Insulin antibody [8E2] (HRP) (ab24455) 使用論文

ab24455 has not yet been referenced specifically in any publications.

Product Wall

Merci d'avoir pris le temps de répondre à notre questionnaire.

Je suis désolé de ne pas avoir pu résoudre correctement le problème que vous avez rencontré avec l'anticorps anti-insuline ab24455...

Read More

Thank you for contacting us.

In our we recommend carbonate/bicarbonate buffer at pH 9.6. However, the capture antibody can also be diluted with PBS, pH 7.4, and the HRP-conjuga...

Read More
Application ELISA
Sample Mouse Purified protein (Insulin standards)
Specification Insulin standards
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 37°C
Type Sandwich (Detection)

Abcam user community

Verified customer

投稿 Oct 10 2012

Thank you for your reply.
Please keep in mind that mouse has not been tried and we do not know if and how well the antibodies will work with this species.
I'll pass the lot numbers to the lab.

I hope this information is helpful to you....

Read More

Thank you for your phone call.
I have contacted the lab for more information about antibody dilutions and for a detailed protocol, and will let you know when I hear back.
Below, please find the discount codes for trying these antibodies with ...

Read More

For ab24455 DISCOUNT CODE: ***

For ab1965 DISCOUNT CODE: ***

Expiration date: December 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab1965 and ab24455 for useon mouse samples. Each code...

Read More

Thank you for yoiru enquiry.

I can confirm that these two antibodies have been tested together in Sandwich ELISA system. The storage instructions can be found on the on-line product datasheets. We normally suggest our customers using the prod...

Read More

Merci pour votre réponse.

J'ai mis en place l'envoi de 4 unités de ab24455. Le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmation comprenant les d&eacu...

Read More

Je suis vraiment désolé que le dernier lot GR73820 ne soit pas, une fois de plus, de bonne qualité.

C'est malheureusement le seul lot que nous avons actuellement en stock. Je pensais en vous contactant la semaine derni&eg...

Read More

Thank you for your response.

You have raised an excellent point that I will bring to our datasheet team. While we do list sandwich ELISA as a different application perhaps we could list the specific ELISA type as we do for IHC. In most cases ...

Read More