製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to INSM1
  • 特異性
    Ab30940 recognises INSM1.
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide derived from residues 393 - 442 of human INSM1.

    .

  • ポジティブ・コントロール
    • Fetal lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21609490Cells fixed with 4% PFA, treated with 0.5% Triton for 5 minutes at Room Temperature.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/7.8125e+006.

ターゲット情報

  • 関連性
    INSM1 may be associated with the transformation of neuroendocrine cells. It is found in several tumor cell lines of neuroendocrine origin including pheochromocytoma, medullary thyroid carcinoma, insulinoma, pituitary tumor, and small cell lung carcinoma. It is not found in any of the normal tissues tested.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • IA1 antibody
    • Insulinoma associated protein 1 antibody
    • Zinc finger protein IA1 antibody

画像

  • Anti-INSM1 antibody (ab30940) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + fetal lung lysate at 10 µg/ml

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

参考文献

This product has been referenced in:
  • Kamakura M  et al. Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0. Virol J 8:257 (2011). ICC/IF, WB . Read more (PubMed: 21609490) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain 2 day-old mouse)
Loading amount
20 µg
Specification
Brain 2 day-old mouse
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Jun 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録