製品の概要

  • 製品名Anti-INSM1 antibody
    INSM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to INSM1
  • 特異性Ab30940 recognises INSM1.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide derived from residues 393 - 442 of human INSM1.

    .

  • ポジティブ・コントロール
    • Fetal lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21609490Cells fixed with 4% PFA, treated with 0.5% Triton for 5 minutes at Room Temperature.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/7.8125e+006.

ターゲット情報

  • 関連性INSM1 may be associated with the transformation of neuroendocrine cells. It is found in several tumor cell lines of neuroendocrine origin including pheochromocytoma, medullary thyroid carcinoma, insulinoma, pituitary tumor, and small cell lung carcinoma. It is not found in any of the normal tissues tested.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • IA1 antibody
    • Insulinoma associated protein 1 antibody
    • Zinc finger protein IA1 antibody

Anti-INSM1 antibody 画像

  • Anti-INSM1 antibody (ab30940) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + fetal lung lysate at 10 µg/ml

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

Anti-INSM1 antibody (ab30940) 使用論文

This product has been referenced in:
  • Kamakura M  et al. Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0. Virol J 8:257 (2011). ICC/IF, WB . Read more (PubMed: 21609490) »
  • Kamakura M  et al. Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0. Virol J 8:257 (2011). WB, ICC/IF . Read more (PubMed: 21609490) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brain 2 day-old mouse)
Loading amount 20 µg
Specification Brain 2 day-old mouse
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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投稿 Jun 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"