Anti-Insig2 抗体 (ab86415)

製品の概要

  • 製品名Anti-Insig2 antibody
    Insig2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Insig2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to a region within amino acids 72-121 (

    WVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHA

    ) of Human Insig2 (NP_057217)

  • ポジティブ・コントロール
    • Human fetal muscle lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性Insig2 is highly similar to the protein product encoded by gene Insig1. Both Insig1 protein and Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi.
  • 細胞内局在Endoplasmic reticulum
  • 参照データベース
  • 別名
    • Hypothetical protein INSIG2 antibody
    • INSIG 2 antibody
    • INSIG 2 membrane protein antibody
    • INSIG2 membrane protein antibody
    • Insulin induced gene 2 antibody
    • Insulin induced gene 2 protein antibody
    • Insulin induced protein 2 antibody
    • MGC26273 antibody
    see all

Anti-Insig2 antibody 画像

  • Anti-Insig2 antibody (ab86415) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : ~40 kDa,~90 kDa. We are unsure as to the identity of these extra bands.

Anti-Insig2 antibody (ab86415) 使用論文

ab86415 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (LIVER)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 17 2016

Application Western blot
Sample Rat Tissue lysate - whole (rat kidney lysate)
Loading amount 140 µg
Specification rat kidney lysate
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 13 2012

Thank you for sending the protocol and sample details. The blot result is interesting, if you loaded the same amount of protein per gel lane, given the intensity of the signal in the plant extract lane, but I do not have an explanation for why the anti...

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Thank you for sending the details of your western blot protocol.
The Insig2 protein is expected to run to about 25 kDa in SDS-PAGE gels. Are you including a a reducing agent in the loading buffer, and boiling to denature the samples?

Ca...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (nerve)
Loading amount 20 µg
Specification nerve
Gel Running Conditions Reduced Denaturing (12% SDS)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Xinpeng Dun

Verified customer

投稿 Jul 04 2012

DISCOUNT CODE: ***
Expiration date: September 9, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab125186 using mouse or rat samplesand include this cod...

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Thank you for your reply and for the additional information.

I am sending a vial of ab86415 free of charge on the order ***. This antibody is in stock and should arrive on Monday. Please keep me updated about how this antibody works in your W...

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Thank you for contacting us and for letting us know about the trouble with ab86351. I am sorry to see that the results have been so poor.

I would be happy to send a vial of ab86415 as a replacement. Do you have your original order or PO numbe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"