Anti-Insig1 抗体 (ab70784)
Key features and details
- Rabbit polyclonal to Insig1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Insig1 antibody -
製品の詳細
Rabbit polyclonal to Insig1 -
由来種
Rabbit -
特異性
ab70784 recognizes ~30 kDa InSig1 and its precursor (~50 kDa). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3854 Insig1 Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.03% Proclin 300
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine), PBS, 0.15% EDTA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70784の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 30, 50 kDa (predicted molecular weight: 30, 50 kDa).
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特記事項 |
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 30, 50 kDa (predicted molecular weight: 30, 50 kDa). |
ターゲット情報
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機能
Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. -
組織特異性
Expressed in all tissues tested with highest expression in the liver. -
配列類似性
Belongs to the INSIG family. -
翻訳後修飾
Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3638 Human
- Omim: 602055 Human
- SwissProt: O15503 Human
- Unigene: 520819 Human
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別名
- CL 6 antibody
- CL6 antibody
- INSI1_HUMAN antibody
see all
画像
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Anti-Insig1 antibody (ab70784) at 0.5 µg/ml + Jurkat cell lysate
Predicted band size: 30, 50 kDa
Observed band size: 30,50 kDa why is the actual band size different from the predicted?
~30 kDa band is InSig1; ~50 kDa band is its precursor.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab70784 は 16 報の論文で使用されています。
- Hu M et al. The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1. Int J Biol Sci 18:3107-3121 (2022). PubMed: 35637957
- Tiong TY et al. Targeting the SREBP-1/Hsa-Mir-497/SCAP/FASN Oncometabolic Axis Inhibits the Cancer Stem-like and Chemoresistant Phenotype of Non-Small Cell Lung Carcinoma Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35806291
- Zhong S et al. Acetaldehyde Dehydrogenase 2 regulates HMG-CoA reductase stability and cholesterol synthesis in the liver. Redox Biol 41:101919 (2021). PubMed: 33740503
- Tao M et al. LPCAT1 reprogramming cholesterol metabolism promotes the progression of esophageal squamous cell carcinoma. Cell Death Dis 12:845 (2021). PubMed: 34518524
- Menzies SA et al. The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. Elife 7:N/A (2018). PubMed: 30543180