Anti-INSC 抗体 (ab102953)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to INSC
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region between amino acids 416-446 from the central region of Human INSC (UniProt ID: Q1MX18).

  • ポジティブ・コントロール
    • WiDr cell line lysate; Hepatocarcinoma tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 63 kDa.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    May function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. Involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium.
  • 組織特異性
    Isoform 1 is expressed in various tissues with stronger expression in liver, kidney and small intestine. Isoform 2 is abundantly expressed in small intestine and to a lower extent in lung and pancreas.
  • 発生段階
    Expressed in fetal cochlea.
  • 細胞内局在
    Cytoplasm. Localizes to the apical cortex of mitotic epidermal cells and to the apical side of neuronal progenitor cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Insc antibody
    • INSC_HUMAN antibody
    • Inscuteable homolog (Drosophila) antibody
    • Protein inscuteable homolog antibody

画像

  • ICC/IF image of ab102953 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102953 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-INSC antibody (ab102953) at 1/100 dilution + WiDr cell line lysate at 35 µg

    Predicted band size : 63 kDa
  • Immunohistochemical analysis of INSC in formalin fixed and paraffin embedded hepatocarcinoma tissue using ab102953 at a 1/50 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.

参考文献

ab102953 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse Embryo (day 8.5))
Specification
Mouse Embryo (day 8.5)
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Derek Passer

Verified customer

投稿 Oct 27 2011

Thanks for your call today and your interest in our INSC antibodies. DISCOUNT CODES: ab102953: *** ab107096: *** Expiration Date: February 14, 2012 Since these antibodies have not been tested in IHC-Fr on ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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