Anti-iNOS 抗体 [NOS-IN] (ab49999)
Key features and details
- Mouse monoclonal [NOS-IN] to iNOS
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG1
製品の概要
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製品名
Anti-iNOS antibody [NOS-IN]
iNOS 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [NOS-IN] to iNOS -
由来種
Mouse -
特異性
This antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.
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アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Mouse iNOS aa 1126-1144 conjugated to keyhole limpet haemocyanin.
Sequence:FSYGAKKGSALEEPKATRL
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ポジティブ・コントロール
- WB: Whole cell extract of RAW 264.7 activated with LPS and IFN-gamma. ICC/IF: RAW 264.7 activated with LPS and IFN-gamma.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.087% Sodium azide
Constituent: 2.93% PBS -
Concentration information loading...
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特記事項(精製)
This antibody is purified from hybridoma cell culture. -
ポリ/モノ
モノクローナル -
クローン名
NOS-IN -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab49999の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa. |
ターゲット情報
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機能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
組織特異性
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
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参照データベース
- Entrez Gene: 18126 Mouse
- SwissProt: P29477 Mouse
- Unigene: 2893 Mouse
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別名
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence analysis of IFN-γ/LPS-activated RAW-264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling iNOS with ab49999 at 1/2000 dilution.
Cells were fixed and permeabilized with 4% paraformaldehyde and 0.5% Triton™ X-100. The antibody was developed using Goat anti-Mouse IgG, Cy3-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
This image was generated using the ascites version of the product.
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Lane 1 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/500 dilution
Lane 2 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/1000 dilution
Lane 3 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/2500 dilution
Lane 4 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/5000 dilution
All lanes : IFN-gamma/LPS-activated RAW-264.7 cell extract
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase and chemiluminescent substrate
Predicted band size: 131 kDaThis image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (79)
ab49999 は 79 報の論文で使用されています。
- Ma Y et al. Photobiomodulation Attenuates Neurotoxic Polarization of Macrophages by Inhibiting the Notch1-HIF-1α/NF-κB Signalling Pathway in Mice With Spinal Cord Injury. Front Immunol 13:816952 (2022). PubMed: 35371065
- Fan Z et al. Intestinal Flora Changes Induced by a High-Fat Diet Promote Activation of Primordial Follicles through Macrophage Infiltration and Inflammatory Factor Secretion in Mouse Ovaries. Int J Mol Sci 23:N/A (2022). PubMed: 35563189
- Fan H et al. Exosomes derived from olfactory ensheathing cells provided neuroprotection for spinal cord injury by switching the phenotype of macrophages/microglia. Bioeng Transl Med 7:e10287 (2022). PubMed: 35600663
- Hang Z et al. Iguratimod Attenuates Macrophage Polarization and Antibody-Mediated Rejection After Renal Transplant by Regulating KLF4. Front Pharmacol 13:865363 (2022). PubMed: 35614941
- Carrascal L et al. Acute Colon Inflammation Triggers Primary Motor Cortex Glial Activation, Neuroinflammation, Neuronal Hyperexcitability, and Motor Coordination Deficits. Int J Mol Sci 23:N/A (2022). PubMed: 35628158