Anti-iNOS 抗体 [EPR16635] (ab178945)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16635] to iNOS
- Suitable for: Indirect ELISA, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-iNOS antibody [EPR16635]
iNOS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16635] to iNOS -
由来種
Rabbit -
特異性
This antibody shows low affinity on human samples.
Based on our preliminary data, this antibody is not suitable for THP1 (Human monocytic leukemia monocyte) cell lines in WB.
-
アプリケーション
適用あり: Indirect ELISA, WB, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: RAW 264.7 treated with 0.1 µg/mL LPS for 6 hours, HepG2 treated with 10 µg/mL LPS for 6 hours,whole cell lysates; Human fetal brain lysate; L6 treated with 50 ng/ml IL-1 beta, 20 ng/ml TNF-alpha and 100U/ml IFN-gamma for 24 h, whole cell lysate; ICC/IF: RAW 264.7 cells treated with LPS (0.1 µg/mL), for 6 hours. IP: RAW 264.7 whole cell lysate treated with 1µg/mL LPS for 24h.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16635 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Alexa Fluor® 647 Anti-iNOS antibody [EPR16635] (ab209027)
- Alexa Fluor® 555 Anti-iNOS antibody [EPR16635] (ab209594)
- Alexa Fluor® 568 Anti-iNOS antibody [EPR16635] (ab209595)
- Anti-iNOS antibody [EPR16635] - Mouse IgG2b (Chimeric) (ab210823)
- Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab178945の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Indirect ELISA |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/1000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml). |
ICC/IF | (1) |
1/500.
To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml). |
IP |
1/100.
To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml). |
特記事項 |
---|
Indirect ELISA
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa). To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml). |
ICC/IF
1/500. To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml). |
IP
1/100. To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml). |
ターゲット情報
-
機能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
組織特異性
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
-
参照データベース
- Entrez Gene: 4843 Human
- Entrez Gene: 18126 Mouse
- Entrez Gene: 24599 Rat
- Omim: 163730 Human
- SwissProt: P35228 Human
- SwissProt: P29477 Mouse
- SwissProt: Q06518 Rat
- Unigene: 709191 Human
see all -
別名
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
画像
-
All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/1000 dilution
Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : Mouse colon cancer tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 180 secondsiNOS is not normally expressed in the brain, but can be induced in the brain after inflammatory, infectious, or other damages (PMID: 11138926, PMID: 16156895, PMID: 10322315).
-
All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/1000 dilution
Lane 1 : Untreated L6 (rat skeletal muscle myoblast) whole cell lysate
Lane 2 : L6 treated with 50 ng/ml IL-1 beta, 20 ng/ml TNF-alpha and 100U/ml IFN-gamma for 24 h, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 131 kDa -
ELISA analysis of House mouse iNOS recombinant protein at 1000 ng/mL with ab178945. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
-
All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/20000 dilution
Lane 1 : Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 whole cell lysate treated with 0.1 µg/mL LPS for 6 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
-
Immunocytochemistry/Immunofluorescence analysis of RAW 264.7 non-treated and LPS treated (0.1 µg/mL) cells labelling iNOS with ab178945 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, Alexa Fluor® 594 conjugated anti-alpha tubulin (1/200). Nuclei counterstained with DAPI (blue).
Secondary antibody only controls performed on non-treated and treated cells.
-
iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate treated with 1 µg/ml LPS for 24h with ab178945 at 1/100 dilution.
Lane 1: RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 24h,10ug (Input).
Lane 2: ab178945 IP in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 24h.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab178945 in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 24h.
Western blot was performed from the immunoprecipitate using ab178945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
-
All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/500 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 10ug/ml lipopolysaccharides for 6 hours whole cell lysates
Lane 3 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lane 4 : THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml lipopolysaccharides for 3 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 131 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (190)
ab178945 は 190 報の論文で使用されています。
- Huang Y et al. Pretreatment of the ROS Inhibitor Phenyl-N-tert-butylnitrone Alleviates Sleep Deprivation-Induced Hyperalgesia by Suppressing Microglia Activation and NLRP3 Inflammasome Activity in the Spinal Dorsal Cord. Neurochem Res 48:305-314 (2023). PubMed: 36104611
- Wang Z et al. Jujuboside A attenuates sepsis-induced cardiomyopathy by inhibiting inflammation and regulating autophagy. Eur J Pharmacol 947:175451 (2023). PubMed: 36502962
- Li S et al. Puerarin improves diabetic wound healing via regulation of macrophage M2 polarization phenotype. Burns Trauma 10:tkac046 (2022). PubMed: 36568527
- Yang G & Shi J miRNA-130a-3p targets sphingosine-1-phosphate receptor 1 to activate the microglial and astrocytes and to promote neural injury under the high glucose condition. Open Med (Wars) 17:2117-2129 (2022). PubMed: 36582210
- Gao ZK et al. Pre-ischemic exercise prevents inflammation and apoptosis by inhibiting MAPK pathway in ischemic stroke. Transl Neurosci 13:495-505 (2022). PubMed: 36636513