製品の概要

  • 製品名Anti-iNOS antibody
    iNOS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to iNOS
  • 特異性This antibody detects endogenous levels of total iNOS protein.
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human iNOS. Immunogen is in the range of aa 1101-1150
    Database link: P35228

  • ポジティブ・コントロール
    • Human lung carcinoma tissue and extracts from NIH/3T3 cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab53769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
  • 組織特異性Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
  • 配列類似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HEP-NOS antibody
    • Hepatocyte NOS antibody
    • HEPNOS antibody
    • inducible antibody
    • Inducible nitric oxide synthase antibody
    • Inducible NO synthase antibody
    • Inducible NOS antibody
    • iNOS antibody
    • MAC NOS antibody
    • Macrophage NOS antibody
    • Nitric oxide synthase 2 inducible antibody
    • Nitric oxide synthase 2 inducible macrophage antibody
    • nitric oxide synthase 2A (inducible, hepatocytes) antibody
    • Nitric oxide synthase antibody
    • Nitric oxide synthase inducible antibody
    • nitric oxide synthase, macrophage antibody
    • NOS 2 antibody
    • NOS antibody
    • Nos II antibody
    • NOS type II antibody
    • nos2 antibody
    • NOS2_HUMAN antibody
    • NOS2A antibody
    • NOS2A, Inducible, Hepatocyte antibody
    • Peptidyl-cysteine S-nitrosylase NOS2 antibody
    see all

Anti-iNOS antibody 画像

  • All lanes : Anti-iNOS antibody (ab53769) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells
    Lane 2 : Extracts from NIH/3T3 cells, plus peptide


    Predicted band size : 131 kDa
    Observed band size : 131 kDa
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53769 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with peptide.

Anti-iNOS antibody (ab53769) 使用論文

This product has been referenced in:
  • McAdam E  et al. Inducible Nitric Oxide Synthase (iNOS) and Nitric Oxide (NO) are Important Mediators of Reflux-induced Cell Signalling in Esophageal Cells. Carcinogenesis : (2012). IHC . Read more (PubMed: 22826608) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Treatment 0.1 ug/ml LPS 24 h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 12 2014

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.
 
Ich habe die Homologie der Immunogene unsere Anti iNOS Antikörper mit Schwein verglichen und habe folgende mögliche Antikörper für Sie:
ab129372 : 85%
ab13691...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"