製品の概要

  • 製品名
    Anti-Inhibin beta A antibody
    Inhibin beta A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Inhibin beta A
  • アプリケーション
    適用あり: ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human, Recombinant fragment
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Inhibin beta A (N terminal). A synthetic peptide corresponding to N-terminal residues of human INHBA (Inhibin beta A chain precursor)
    Database link: P08476

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab56057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.Can be blocked with Inhibin beta A peptide (ab215854). This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • 配列類似性
    Belongs to the TGF-beta family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activin beta-A chain antibody
    • EDF antibody
    • Erythroid differentiation factor antibody
    • Erythroid differentiation protein antibody
    • Follicle stimulating hormone releasing protein antibody
    • FRP antibody
    • FSH releasing protein antibody
    • INHBA antibody
    • INHBA_HUMAN antibody
    • Inhibin beta A chain antibody
    • Inhibin beta A subunit antibody
    • Inhibin, beta 1 antibody
    • Inhibin, beta A (activin A, activin AB alpha polypeptide) antibody
    see all

画像

  • ab56057 staining Inhibin in human testicular cancer (seminoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution and blocked for 20 minutes at 26°C; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-Inhibin beta A antibody (ab56057) at 1/1000 dilution + Human COV434 whole cell lysate at 60 µg

    Secondary
    IRDye® 680LT-conjugated goat anti-rabbit IgG polyclonal at 1/25000 dilution

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Francesco Elia Marino

    See Abreview

  • ab56057 staining Inhibin beta A in human COV434 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with ethanol and blocked for 3 hours at 26°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 21°C. A IRDye® 800CW-conjugated goat anti-rabbit IgG (H+L) polyclonal (1/15000) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

投稿 Jan 16 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Odyssey Blocking Buffer as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample
Human Cell (COV434)
Specification
COV434
Permeabilization
Yes - Ethanol
Fixative
Formaldehyde
Username

Dr. Francesco Elia Marino

Verified customer

投稿 May 28 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
CAS Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Sample
Human Tissue sections (Human testis)
Specification
Human testis
Permeabilization
No
Fixative
Bouin's
Username

Dr. Francesco Elia Marino

Verified customer

投稿 May 12 2014

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Non-reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (COV434 Cell Line)
Specification
COV434 Cell Line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Username

Dr. Francesco Elia Marino

Verified customer

投稿 Mar 12 2014

Thank you for your enquiry and your interest in our products. Unit size, price, availability, tested applications and species can be found on the on-line datasheets: http://www.abcam.com/index.html?datasheet=4241 http://www.abcam.com/index.html...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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