Anti-Inhibin alpha 抗体 [R1] (ab14087)

製品の概要

  • 製品名Anti-Inhibin alpha antibody [R1]
    Inhibin alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [R1] to Inhibin alpha
  • アプリケーション適用あり: WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Human Inhibin alpha aa 233-264. Note that this peptide corresponds to the N-terminus of the mature region of the human inhibin alpha subunit.
    Sequence:

    STPLMSWPWSPSALRLLQRPPEEPAAHANCHR


    Database link: P05111

  • ポジティブ・コントロール
    • human ovary, testis, placenta
  • 特記事項


    Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.08% Sodium Azide
    Constituents: Tissue Culture Supernatant
  • 精製度Tissue culture supernatant
  • 一次抗体 備考Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours.
  • ポリ/モノモノクローナル
  • クローン名R1
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/200. Predicted molecular weight: 32 kDa.
IHC-Fr 1/1 - 1/50.
IHC-P 1/1 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Neat - 1/1/50. Sodium citrate pH 6.0 is recommended for antigen retrieval.

ターゲット情報

  • 機能Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • 組織特異性Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.
  • 配列類似性Belongs to the TGF-beta family.
  • 翻訳後修飾Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A inhibin subunit antibody
    • A inhibin subunit precursor antibody
    • AW55578 antibody
    • IHA antibody
    • inhA antibody
    • INHA_HUMAN antibody
    • Inhibin alpha chain antibody
    • Inhibin alpha chain precursor antibody
    • Inhibin alpha subunit antibody
    see all

Anti-Inhibin alpha antibody [R1] 画像

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of paraffin embedded human ovary tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue (showing sertoli cells, leydig cells and spermatogonia) labeling inhibin alpha with ab14087.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

Anti-Inhibin alpha antibody [R1] (ab14087) 使用論文

ab14087 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Ovary (abnormal))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween 20, pH 6.0
Permeabilization No
Specification Ovary (abnormal)
Blocking step ab127055 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 21°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 17 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"