製品の概要

  • 製品名Anti-Inhibin alpha antibody [EPY2782]
    Inhibin alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPY2782] to Inhibin alpha
  • 特異性Note: 20/23 kDa bands consist of the mature alpha chain. 50/53 kDa bands are the entire proprotein.
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Inhibin alpha aa 1-100 (N terminal).
    Database link: P05111

  • ポジティブ・コントロール
    • Human Adrenal gland, HeLa, Human placenta and LNCaP lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/300.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
    • 組織特異性Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.
    • 配列類似性Belongs to the TGF-beta family.
    • 翻訳後修飾Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A inhibin subunit antibody
      • A inhibin subunit precursor antibody
      • AW55578 antibody
      • IHA antibody
      • inhA antibody
      • INHA_HUMAN antibody
      • Inhibin alpha chain antibody
      • Inhibin alpha chain precursor antibody
      • Inhibin alpha subunit antibody
      see all

    Anti-Inhibin alpha antibody [EPY2782] 画像

    • All lanes : Anti-Inhibin alpha antibody [EPY2782] (ab81322) at 1/2000 dilution

      Lane 1 : Human Adrenal gland lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human placenta lysate
      Lane 4 : LNCaP lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti rabbit at 1/2000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40,50 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

    Anti-Inhibin alpha antibody [EPY2782] (ab81322) 使用論文

    This product has been referenced in:
    • Han L  et al. Characterization of the Mechanism of Inhibin a-Subunit Gene in Mouse Anterior Pituitary Cells by RNA Interference. PLoS One 8:e74596 (2013). Mouse . Read more (PubMed: 24098340) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"