製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ING3
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide designed within residues: MDQLEQRVSE FFMNAKKNKP EWREEQMASI KKDYYKALED ADEKVQLANQ , corresponding to N terminal amino acids 36-85 of Human ING3 (NP_06944)

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    Function: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. Tissue specificity: Expressed in brain, heart, kidney, liver, lung, ovaries, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Disease: Squamous cell carcinoma of the head and neck Similarity: Belongs to the ING family. Contains 1 PHD-type zinc finger. Domain: The PHD-type zinc finger mediates the binding to H3K4me3.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • 1300013A07Rik antibody
    • Eaf 4 antibody
    • Eaf4 antibody
    • FLJ20089 antibody
    • ING 2 antibody
    • ING 3 antibody
    • ING2 antibody
    • ING3 antibody
    • ING3_HUMAN antibody
    • Inhibitor of growth family member 3 antibody
    • Inhibitor of growth protein 3 antibody
    • MEAF4 antibody
    • p47 ING 3 antibody
    • p47 ING3 antibody
    • p47 regulator protein antibody
    • p47ING3 antibody
    • p47ING3 protein antibody
    see all

画像

  • Anti-ING3 antibody (ab84645) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling ING3 with ab84645 at 5µg/ml.

参考文献

ab84645 has not yet been referenced specifically in any publications.

レビューと Q&A

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande de remplacemen...

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Merci d'avoir pris le temps de répondre à nos questions et de nous avoir contactés. Je suis désolée d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab84645.
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Merci d'avoir pris le temps de nous envoyer votre protocole. Je suis désolée d'apprendre qu’ab84645 ne vous donne pas de bons résultats en western blot.

Notre politique est d'offrir tout d'abord la meilleure assista...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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