製品の概要

  • 製品名Anti-ING2 antibody [EPR4597]
    ING2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4597] to ING2
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment (Human)

  • ポジティブ・コントロール
    • WB: T47-D, HepG2 or HT-1080 cell lysates IHC-P: Human kidney tissue This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IP 1/10 - 1/100.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
    • 組織特異性Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
    • 配列類似性Belongs to the ING family.
      Contains 1 PHD-type zinc finger.
    • ドメインThe PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).
    • 細胞内局在Nucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ING 1 like tumor suppressor protein antibody
      • ING 1L antibody
      • ING 2 antibody
      • ING1 like tumor suppressor protein antibody
      • ING1L antibody
      • ING1Lp antibody
      • ING2 antibody
      • ING2_HUMAN antibody
      • Inhibitor of growth 1 like antibody
      • Inhibitor of growth 1 like protein antibody
      • Inhibitor of growth 1-like protein antibody
      • Inhibitor of growth family member 1 like antibody
      • Inhibitor of growth family member 2 antibody
      • Inhibitor of growth protein 2 antibody
      • p32 antibody
      • p33 ING2 antibody
      • p33ING2 antibody
      see all

    Anti-ING2 antibody [EPR4597] 画像

    • ICC/IF image of ab109504 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109504 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-ING2 antibody [EPR4597] (ab109504) at 1/10000 dilution

      Lane 1 : T47-D cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 33 kDa
    • Immunohistochemical analysis of ING2 expression in formalin-fixed, paraffin-embedded Human kidney tissue using ab109504 at 1/1000 dilution.

    Anti-ING2 antibody [EPR4597] (ab109504) 使用論文

    ab109504 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109504.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"