製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ING2
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human ING2.

  • ポジティブ・コントロール
    • WB: HT1080, HEK293, Caco2 and A498 whole cell lysates. ICC/IF: HCT116 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab107703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Abcam recommends using milk as the blocking agent - 5%.

ターゲット情報

  • 機能
    Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
  • 組織特異性
    Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
  • 配列類似性
    Belongs to the ING family.
    Contains 1 PHD-type zinc finger.
  • ドメイン
    The PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).
  • 細胞内局在
    Nucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ING 1 like tumor suppressor protein antibody
    • ING 1L antibody
    • ING 2 antibody
    • ING1 like tumor suppressor protein antibody
    • ING1L antibody
    • ING1Lp antibody
    • ING2 antibody
    • ING2_HUMAN antibody
    • Inhibitor of growth 1 like antibody
    • Inhibitor of growth 1 like protein antibody
    • Inhibitor of growth 1-like protein antibody
    • Inhibitor of growth family member 1 like antibody
    • Inhibitor of growth family member 2 antibody
    • Inhibitor of growth protein 2 antibody
    • p32 antibody
    • p33 ING2 antibody
    • p33ING2 antibody
    see all

画像

  • All lanes : Anti-ING2 antibody (ab107703) at 1 µg/ml (Milk blocking - 5%)

    Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 62 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab107703 stained HCT116 cells. The cells were 4% paraformladehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107703, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab107703 has not yet been referenced specifically in any publications.

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