Anti-Influenza Virus NS1A Binding Protein 抗体 (ab101278)

製品の概要

  • 製品名Anti-Influenza Virus NS1A Binding Protein antibody
    Influenza Virus NS1A Binding Protein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Influenza Virus NS1A Binding Protein
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen corresponds to a region within amino acids 50-100 of Human Influenza Virus NS1A Binding Protein (NP_006460.2).

  • ポジティブ・コントロール
    • HeLa, 293T and NIH/3T3 whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab101278 was affinity purified using an epitope specific to Influenza Virus NS1A Binding Protein immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性NS1ABP plays a role in cell division and in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats and is known to protect cells from cell death induced by actin destabilization. Interacts with NS/Influenza virus NS1A protein and is believed to mediate the inhibition of its splicing activity. It is usually associated with actin filaments (related to speckle domains) but following Influenza Avirus infection, NS1ABP presents a more diffuse distribution in the nucleoplasm
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • Aryl hydrocarbon receptor associated 3 antibody
    • DKFZp686K06216 antibody
    • FLARA 3 antibody
    • FLARA3 antibody
    • HSPC068 antibody
    • IVNS1ABP antibody
    • IVNS1ABP protein antibody
    • KIAA0850 antibody
    • NCX downstream gene 1 antibody
    • ND 1 antibody
    • ND1 antibody
    • NS 1 antibody
    • NS1 antibody
    • NS1 binding protein antibody
    • NS1 binding protein like protein antibody
    • NS1 BP antibody
    • NS1BP antibody
    see all

Anti-Influenza Virus NS1A Binding Protein antibody 画像

  • All lanes : Anti-Influenza Virus NS1A Binding Protein antibody (ab101278) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 71 kDa


    Exposure time : 3 minutes
  • Detection of Influenza Virus NS1A Binding Protein by Western Blot of Immunprecipitate.
    ab101278, at 1 µg/ml, staining Influenza Virus NS1A Binding Protein in HeLa whole cell lysate immunoprecipitated using ab101278 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-Influenza Virus NS1A Binding Protein antibody (ab101278) 使用論文

ab101278 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH 3T3 J2)
Permeabilization Yes - 0,15% Triton X-100, 5 min
Specification NIH 3T3 J2
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Simon Boudreault

Verified customer

投稿 Dec 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"