Anti-Influenza A Virus Nucleoprotein 抗体 [IV F8] (ab8261)

製品の概要

  • 製品名Anti-Influenza A Virus Nucleoprotein antibody [IV F8]
    Influenza A Virus Nucleoprotein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [IV F8] to Influenza A Virus Nucleoprotein
  • 特異性This antibody reacts with nucleoprotein (NP), which is common for Influenza A(H1N1), A(H3N2) and other Influenza A viruses.
  • アプリケーション適用あり: ELISA, ICCmore details
  • 免疫原

    Purified influenza virus type A strain H1N1.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody is affinity purified.
  • ポリ/モノモノクローナル
  • クローン名IV F8
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
ICC
  • 追加情報ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.

    Can also be used for detection and serotyping of influenza virus type A.
    Inhibit viral reproduction after fatty acid acylation.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    ICC protocol:
    1)Cell monolayer was grown on a glass and infected with the virus for 2 days at 37 oC.
    2)Glass was dried and the cells were fixed with cold acetone at 4 oC for 10 min.
    3)Ab8261 was introduced in concentrations from 1 to 100 µg/ml in PBS and incubated for 30 min in RT.
    4)Glass was washed with PBS several times and incubated with goat anti-mouse IgG labeled with FITC.
  • ターゲット情報

    • 関連性The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
    • 細胞内局在Host cell nucleus
    • 別名
      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all

    Anti-Influenza A Virus Nucleoprotein antibody [IV F8] (ab8261) 使用論文

    ab8261 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for getting back to me. The concentration of ab8261 needs to be determined by the end user. I would recommend that you perform a series of dilutions and adjust your future experiments thereof. We have some comprehensive ELISA protocols...

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    Thank you for your email. Looking at the datasheet for ab5416, it states that the antibody has been tested for application in Western blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. To our knowledge, it has not yet been test...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"