Anti-Influenza A Virus Nucleoprotein 抗体 [C43] (ab128193)
Key features and details
- Mouse monoclonal [C43] to Influenza A Virus Nucleoprotein
- Suitable for: WB, ELISA, ICC/IF, Flow Cyt
- Reacts with: Influenza A
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
-
製品名
Anti-Influenza A Virus Nucleoprotein antibody [C43]
Influenza A Virus Nucleoprotein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [C43] to Influenza A Virus Nucleoprotein -
由来種
Mouse -
特異性
Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34).
No cross reactivity with influenza B viruses.
-
アプリケーション
適用あり: WB, ELISA, ICC/IF, Flow Cytmore details -
種交差性
交差種: Influenza A -
免疫原
Tissue, cells or virus corresponding to Influenza A Virus Nucleoprotein. Human Influenza A Virus H3N2 strain
Database link: P69291 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
-
精製度
Proprietary Purification -
特記事項(精製)
Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE -
ポリ/モノ
モノクローナル -
クローン名
C43 -
アイソタイプ
IgG2a -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128193の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/300 - 1/1000.
|
|
ELISA |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/200.
|
|
Flow Cyt |
Use at an assay dependent concentration. PubMed: 24067955
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
---|
WB
1/300 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
1/200. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 24067955 ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
関連性
The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002). -
細胞内局在
Host cell nucleus -
参照データベース
- Entrez Gene: 956531 Influenza A
- SwissProt: P03466 Influenza A
- SwissProt: P69291 Influenza A
-
別名
- Common flu NP antibody
- Influenza A virus NP antibody
- NP antibody
see all
画像
-
Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. Influenza A Virus Nucleoprotein antibody (ab128193) efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an green flurescent secondary antibody.
-
Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/1000 dilution + MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34
Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution -
Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). Influenza A Virus Nucleoprotein antibody (ab128193) specifically recognized NP-expressing cells while a proprietary mouse anti-HA monoclonal antibody specifically recognized HA.
-
All lanes : Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/300 dilution
Lanes 1 & 6 & 11 : MDCK cells mock infected
Lane 2 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 3 hrs post-infection
Lane 3 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 9 hrs post-infection
Lane 4 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 24 hrs post-infection
Lane 5 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 48 hrs post-infection
Lane 7 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 3 hrs post-infection
Lane 8 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 9 hrs post-infection
Lane 9 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 24 hrs post-infection
Lane 10 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 48 hrs post-infection
Lane 12 : MDCK cells infected H5N1
(A/duck/HK/342/78)collected 3 hrs post-infection
Lane 13 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 9 hrs post-infection
Lane 14 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 24 hrs post-infection
Lane 15 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 48 hrs post-infection
Tubulin was used as loading control.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (33)
ab128193 は 33 報の論文で使用されています。
- Liu HY et al. Single-virus tracking with quantum dots in live cells. Nat Protoc 18:458-489 (2023). PubMed: 36451053
- Wu W et al. MicroRNA let-7 Suppresses Influenza A Virus Infection by Targeting RPS16 and Enhancing Type I Interferon Response. Front Cell Infect Microbiol 12:904775 (2022). PubMed: 35873150
- John SP et al. Small-molecule screening identifies Syk kinase inhibition and rutaecarpine as modulators of macrophage training and SARS-CoV-2 infection. Cell Rep 41:111441 (2022). PubMed: 36179680
- Kelly JN et al. Comprehensive single cell analysis of pandemic influenza A virus infection in the human airways uncovers cell-type specific host transcriptional signatures relevant for disease progression and pathogenesis. Front Immunol 13:978824 (2022). PubMed: 36268025
- Pila-Castellanos I et al. Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy. PLoS Pathog 17:e1009340 (2021). PubMed: 33596274