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Conjugated with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
Our Abpromise guarantee covers the use of ab20921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/5 - 1/20. Acetone fixation of the antigen in a permissive tissue culture system is recommended prior to direct staining.|
|Flow Cyt||Use at an assay dependent concentration.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.