Anti-Influenza A Virus Nucleoprotein 抗体 [431] (FITC) (ab20921)

製品の概要

  • 製品名
    Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC)
    Influenza A Virus Nucleoprotein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [431] to Influenza A Virus Nucleoprotein (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • 特異性
    Specific for nucleoprotein of all Influenza A strains. The following isolates have been tested and found positive: A/Hong Kong/1/68 A/Lenningrad/358/80 A/Sychuan/2/87 A/Zakarpatje/354/89 A/St. Petersburg/40/92 A/Beijing/32-92 A/Swine/1976/31 A/PR/8/34 A/FM/1/86 A/Leningrad/325/88 A/Texas/36/91 A/Taiwan/1/86 A/Leningrad/459/80 A/Hen/Kishinev/75 A/Duck/Alberta/35/76 A/Duck/Ukraine/1/63 A/Duck/California/72
  • アプリケーション
    適用あり: ICC/IF, Flow Cytmore details
  • 種交差性
    Reacts with the Influenza A virus.
  • 免疫原

    Purified virions.

  • 特記事項

    Conjugated with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 10mg/ml BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Ascites
  • 特記事項(精製)
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    431
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/5 - 1/20. Acetone fixation of the antigen in a permissive tissue culture system is recommended prior to direct staining.
Flow Cyt Use at an assay dependent concentration.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性
    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • 細胞内局在
    Host cell nucleus
  • 別名
    • Common flu NP antibody
    • Influenza A virus NP antibody
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody
    • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
    see all

画像

  • ab20921 staining Influenza A Virus Nucleoprotein in the Influenza infected Human B-cell lymphoma cell line by Flow Cytometry. Cells were cultured in PBS + 1% BSA and were fixed and permeabilized using a Kit. The sample was incubated with the primary antibody (1/50 in a wash) for 1 hour at 4°C.
    Gating Strategy: Single cell suspension.

参考文献

This product has been referenced in:
  • Hoffmann J  et al. Viral and bacterial co-infection in severe pneumonia triggers innate immune responses and specifically enhances IP-10: a translational study. Sci Rep 6:38532 (2016). Read more (PubMed: 27922126) »
  • DiLillo DJ  et al. Broadly neutralizing hemagglutinin stalk-specific antibodies require Fc?R interactions for protection against influenza virus in vivo. Nat Med 20:143-51 (2014). Flow Cyt ; Influenza A . Read more (PubMed: 24412922) »

See all 11 Publications for this product

レビューと Q&A

Gern geschehen, jederzeit gerne wieder!

Vielen Dank für Ihren Anruf.

Wie versprochen habe ich im Labor wegen der Lagerbedingungen für den Anti-Influenza A Virus Nucleoprotein- Antikörper ab20921 nachgefragt, und folgendes wurde uns bestätigt:

Die Instr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Influenza infected B-cell lymphoma cell line)
Specification
Influenza infected B-cell lymphoma cell line
Preparation
Cell harvesting/tissue preparation method: Spin & Resuspend
Sample buffer: PBS w/ 1% BSA
Fixation
BD Fix/Perm Kit
Permeabilization
Yes - BD Fix/Perm Kit
Gating Strategy
Single cell suspension
Username

Abcam user community

Verified customer

投稿 Oct 20 2011

Thank you for your enquiry. I am sincerely sorry to confirm that we do not have any data to provide regarding the crossreactivity of ab20921 Influenza A Virus Nucleoprotein antibody with Influenza A H1N1 Brisbane/10 or Brisbane 59. This has not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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