Anti-Influenza A Virus Hemagglutinin H1 抗体 [B219M] (ab66189)


  • 製品名
    Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M]
    Influenza A Virus Hemagglutinin H1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B219M] to Influenza A Virus Hemagglutinin H1
  • 由来種
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    Reacts with Influenza A Virus.
  • 免疫原

    Purified Influenza A/NewCaledonia/20/99 virus (strain H1N1)

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab91531 as a replacement.


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    >90% pure (SDS-PAGE).
  • ポリ/モノ
  • クローン名
  • ミエローマ
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab66189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use a concentration of 5 µg/ml.


  • 関連性
    Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells.
  • 別名
    • HA antibody
    • Hemagglutinin antibody
    • Hemagglutinin HA antibody


  • Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189) at 5 µg/ml + Influenza A/Taiwan/1/86 at 0.5 µg

    HRP-conjugated anti-mouse IgG

    Performed under reducing conditions.

    Protocol notes:

    • SDS-PAGE in reducing conditions, 10 % acrylamide
    • After SDS-PAGE transfer to nitrocellulose membrane
    • Blocking in PBS, containing 0.5% Tween 20, 10% dry milk (w/v) – incubation 10 minutes at room temperature.
    • Primary antibody: 5 µg/ml diluted in PBS containing 0.5% Tween 20 – incubation 1 hour at room temperature.
    • Secondary antibody: HRP-conjugated anti-mouse IgG diluted in PBS, containing 0.5% Tween 20 – incubation 1hour at room temperature.
    • Diaminobenzidine 50 µg/ml – 20 min



This product has been referenced in:
  • Ahmed SR  et al. In situ self-assembly of gold nanoparticles on hydrophilic and hydrophobic substrates for influenza virus-sensing platform. Sci Rep 7:44495 (2017). Read more (PubMed: 28290527) »
  • Price JV  et al. Characterization of influenza vaccine immunogenicity using influenza antigen microarrays. PLoS One 8:e64555 (2013). Read more (PubMed: 23734205) »

See all 3 Publications for this product

レビューと Q&A

Thank you for contacting Abcam. The free of charge replacement of ab66189 with ab119981 has been placed on order xxxx as you have requested. I hope that this information is helpful. Please let me know if you have any questions or there are ot...

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Thanks for the additional information.

It does appear as if these two antibodies are not working as expected in ELISA. I would be happy to replace them for you with any other primary antibodies.

I will just need the order reference ...

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Thanks so much for the additional information.

It looks like ab66189 and ab61986 are being use as detection antibodies which are in turn detected by anti-mouse secondary antibodies? Is this the case?

If so I was wondering how much o...

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Thank you for your enquiry and for completing the questionnaire.

I just have a few follow up questions:

1. Please describe the sample loaded onto well?

2. Did other antibodies work in ELISA assay with this same sample? Read More

I would still need the accession number for your strain, but I just wanted to let you know of additional information about 2 of our products.

Concerning ab61986, this antibody recognizes all Hemagglutinin from H1N1 strains. The immunoge...

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