Ab20030 is purified from the whole antiserum using Sodium sulfate precipitation and ion-exchange chromatography. Care is taken to ensure complete removal of any free fluorescein from the final product.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Direct IF: 1/10 - 1/50.
Direct FA staining of target antigens in a permissive tissue culture system.
Acetone fixation of the antigen source is recommended prior to staining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Influenza A and B are the two types of influenza viruses that cause epidemic human disease. Influenza type C infections cause a mild respiratory illness and are not thought to cause epidemics.
Influenza A viruses are further categorized into subtypes on the basis of two surface antigens: hemagglutinin (H) and neuraminidase (N). Strains are also described by geographic origin, strain number and year of isolation.