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Our Abpromise guarantee covers the use of ab20841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
This product may be used in place of neat antiserum in almost any appropriate antibody-based technique.
|Conjugation||Use at an assay dependent concentration.|
ab20841 staining Influenza A Virus in reconstructed 1918 H1N1 influenza infected Mouse lung tissue sections by Immunohistochemistry. Tissue was fixed with 10% neutral-buffered formalin and embedded in paraffin. Slides were deparaffinized, rehydrated in water, steam antigen retrieved in pH 6 citrate buffer, and blocked with 3% H2O2. Samples were incubated with primary antibody (1/500) for 1 hour at room temperature. Top - EUK-207 treated mouse, 8 days postinfection, Bottom uninfected mouse control. Brown staining indicates positive staining in cell's cytoplasm and/or nucleus. Cellular debris is also frequently positive.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"