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RabMAb

Anti-Indoleamine 2, 3-dioxygenase 抗体 [EPR1230Y] (ab76157)

製品の概要

  • 製品名
    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y]
    Indoleamine 2, 3-dioxygenase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase
  • アプリケーション
    適用あり: ICC/IF, WBmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues within human Indoleamine 2, 3-dioxygenase

  • ポジティブ・コントロール
    • WB: HeLa cell lysate treated with IFN gamma. ICC/IF: HepG2 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB 1/2500 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能
      Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
    • パスウェイ
      Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
    • 配列類似性
      Belongs to the indoleamine 2,3-dioxygenase family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 3-dioxygenase antibody
      • I23O1_HUMAN antibody
      • IDO 1 antibody
      • IDO antibody
      • IDO-1 antibody
      • IDO1 antibody
      • INDO antibody
      • indolamine 2,3 dioxygenase antibody
      • Indole 2 3 dioxygenase antibody
      • indoleamine 2 3 dioxygenase 1 antibody
      • indoleamine 2 3 dioxygenase antibody
      • Indoleamine 2,3-dioxygenase 1 antibody
      • Indoleamine pyrrole 2 3 dioxygenase antibody
      • Indoleamine-pyrrole 2 antibody
      see all

    画像

    • ab76157 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76157 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/5000 dilution + HeLa cell lysate treated with IFN gamma at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

    参考文献

    This product has been referenced in:
    • Takeshita K  et al. Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration. Stem Cell Res Ther 8:101 (2017). WB, ICC/IF ; Human . Read more (PubMed: 28446226) »
    • Yamazaki T  et al. Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in human epithelial HeLa cells treated with interferon-?. J Infect Chemother 20:460-4 (2014). Human . Read more (PubMed: 24855914) »

    See all 3 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Non-reduced Denaturing (gel 10 %)
    Sample
    Dog Cell lysate - whole cell (adipose tissue derived mesencymal stem cell)
    Specification
    adipose tissue derived mesencymal stem cell
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

    Dr. Seunghoon Lee

    Verified customer

    投稿 Sep 12 2013

    Following up my previous message, I received confirmation that, for the IDO antibody ab76157,  no testing has been done on rat samples. I can send this as a replacement but if you would rather have a credit or refund for ab106134, I can issue that inst...

    Read More

    The producer of this clone has provided the following information about the immunogen for ab76157: "Sequence homology of the immunogen with rat IDO is 86%. Species cross-reactivity data seems to be missing, so I will update you soon." It is not c...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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