Anti-Indoleamine 2, 3-dioxygenase 抗体 (ab55305)

製品の概要

  • 製品名Anti-Indoleamine 2, 3-dioxygenase antibody
    Indoleamine 2, 3-dioxygenase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Indoleamine 2, 3-dioxygenase
  • アプリケーション適用あり: IHC-Fr, WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-404 of Human Indoleamine 2, 3-dioxygenase

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 22415306
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
  • パスウェイAmino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
  • 配列類似性Belongs to the indoleamine 2,3-dioxygenase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3-dioxygenase antibody
    • I23O1_HUMAN antibody
    • IDO 1 antibody
    • IDO antibody
    • IDO-1 antibody
    • IDO1 antibody
    • INDO antibody
    • indolamine 2,3 dioxygenase antibody
    • Indole 2 3 dioxygenase antibody
    • indoleamine 2 3 dioxygenase 1 antibody
    • indoleamine 2 3 dioxygenase antibody
    • Indoleamine 2,3-dioxygenase 1 antibody
    • Indoleamine pyrrole 2 3 dioxygenase antibody
    • Indoleamine-pyrrole 2 antibody
    see all

Anti-Indoleamine 2, 3-dioxygenase antibody 画像

  • Indoleamine 2 3-dioxygenase antibody (ab55305) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.


  • Predicted band size : 45 kDa
    Indoleamine 2 3-dioxygenase antibody (ab55305) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab55305 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55305, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Indoleamine 2, 3-dioxygenase antibody (ab55305) 使用論文

This product has been referenced in:
  • Wang X  et al. The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation. Mol Cell Biol 35:598-609 (2015). Flow Cyt ; Mouse . Read more (PubMed: 25452304) »
  • Stapelberg M  et al. Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans. Free Radic Biol Med 67C:41-50 (2013). WB . Read more (PubMed: 24145120) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate Cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Permeabilization No
Specification Prostate Cancer
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Formaldehyde
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投稿 Nov 12 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin melanoma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Permeabilization No
Specification Skin melanoma
Blocking step Dako Endogenous Peroxidase Block as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Nov 02 2015

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I have found a research paper about Indoleamine 2,3-Dioxygenase tissue distribution and cellular localization that may be useful to determine the cellular localization.

Indoleamine 2,3-Dioxygenase Tiss...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"