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All antibodies work in different combinations of applications, species and samples, so we encourage our customers to write a short Abreview, so that future customers can make an informed decision about the usefulness of a particular antibody for their specific experiments.

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review by Rebecca Somers submitted on 9 January 2007

Application:

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

 

MRP2 antibody [M2 III-6] (ab3373)

 

Sample

Rat Tissue sections (Liver)

Specification

Liver

 

Fixative:

Formaldehyde

Antigen retrieval step:

Heat mediated

Blocking step:

Serum as blocking agent for  20 mins  · Concentration: 5%

 

Primary Antibody

Dilution

1/200

Incubation time:

1 hour 

 

Secondary Antibody

Name:

Non-Abcam Antibody was used: Goat anti Mouse Envision
Conjugation: HRP

 

Dilution:

1/1

 

Additional Data

Rating

Good 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

Additional notes

Heat-mediated antigen-retrieval using citrate buffer (0.01M, pH6) using RHS-2 Microwave Rapid Histoprocessor with pressure vessel at 110C for 2 minutes produced best results.

Positive staining also achieved without pretreatment, with enzymic (pronase) or heat-mediated (edta buffer) antigen-retrieval (but more background/less specificity).

Image

MRP2 antibody [M2 III-6] for IHC-P (Rat)

Copyright image used under license