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Application: |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) |
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Sample |
Rat Tissue sections (Liver) |
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Specification |
Liver |
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Fixative: |
Formaldehyde |
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Antigen retrieval step: |
Heat mediated |
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Blocking step: |
Serum as blocking agent for 20 mins · Concentration: 5% |
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Primary Antibody |
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Dilution |
1/200 |
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Incubation time: |
1 hour |
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Secondary Antibody |
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Name: |
Non-Abcam Antibody was used: Goat anti Mouse Envision
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Dilution: |
1/1 |
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Additional Data |
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Rating |
Good
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Additional notes |
Heat-mediated antigen-retrieval using citrate buffer (0.01M, pH6) using RHS-2 Microwave Rapid Histoprocessor with pressure vessel at 110C for 2 minutes produced best results.
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Image |
Copyright image used under license |
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