All products work in different combinations of applications, species and samples, so we encourage our customers to write a short Abreview, so that future customers can make an informed decision about the usefulness of a particular product for their specific experiments.
We calculate the average star ratings from a subset of all our reviews. We use all conclusive reviews (ie from 1 to 5 stars) where the datasheet states that the product has been tested in the application.
Submit an Abreview and earn Abpoints
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Application: |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) |
|||||||||
|
||||||||||
Sample |
Rat Tissue sections (Liver) |
|||||||||
Specification |
Liver |
|||||||||
Fixative: |
Formaldehyde |
|||||||||
Antigen retrieval step: |
Heat mediated |
|||||||||
Blocking step: |
Serum as blocking agent for 20 mins · Concentration: 5% |
|||||||||
|
||||||||||
Other product details |
||||||||||
Dilution |
1/200 |
|||||||||
Incubation time: |
1 hour |
|||||||||
|
||||||||||
Secondary antibody |
||||||||||
Name: |
Non-Abcam antibody was used: Goat anti Mouse Envision
|
|
||||||||
Dilution: |
1/1 |
|||||||||
|
||||||||||
Additional data |
||||||||||
Rating |
Good
|
|||||||||
Additional notes |
Heat-mediated antigen-retrieval using citrate buffer (0.01M, pH6) using RHS-2 Microwave Rapid Histoprocessor with pressure vessel at 110C for 2 minutes produced best results.
|
|||||||||
Image |
Copyright image used under license |
|||||||||
Pricing information loading
review by 
