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Products:Neuroscience >> Neurology process >> Circadian Rhythm
Overview
Product name
Anti-KAT13D / CLOCK antibody
KAT13D / CLOCK 抗体 (9件) 一覧
Description
Rabbit polyclonal to KAT13D / CLOCK
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to a region within amino acids 796-846 of Human KAT13D / CLOCK (NP_004889.1)
Positive control
HeLa and 293T whole cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab93804 was affinity purified using an epitope specific to KAT13D/CLOCK immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Blood >> Blood Pressure regulation
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Neurology process >> Circadian Rhythm
Applications
Show applications key
Our Abpromise guarantee covers the use of ab93804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at a concentration of 2-5 µg/ml.
WB: 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
Tissue specificity
Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.
Cellular localization
Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.
Target information above from: UniProt accessionO15516
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHe8 antibody
- Circadian locomoter output cycles kaput protein antibody
- Circadian locomoter output cycles protein kaput antibody
see all
Database links
- Entrez Gene: 9575 Human
- Omim: 601851 Human
- SwissProt: O15516 Human
- Unigene: 436975 Human
Anti-KAT13D / CLOCK antibody images:
Western blot - KAT13D / CLOCK antibody (ab93804)
All lanes : Anti-KAT13D / CLOCK antibody (ab93804) at 0.1 µg/ml
Lane 1 : HeLa at 50 µg
Lane 2 : HeLa at 15 µg
Lane 3 : HeLa at 5 µg
Lane 4 : 293T at 50 µg
developed using the ECL technique
Predicted band size : 95 kDa
Exposure time : 3 minutes
Immunoprecipitation - KAT13D / CLOCK antibody (ab93804)
ab93804 at 1 µg/ml detecting KAT13D/CLOCK in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of KAT13D/CLOCK
Lane 2: ab93804 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
References for Anti-KAT13D / CLOCK antibody (ab93804)
ab93804 has not yet been referenced specifically in any publications.
Publishing research using ab93804? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"