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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-BMAL1 antibody
BMAL1 抗体 (8件) 一覧
Description
Goat polyclonal to BMAL1
Specificity
ab81974 is expected to recognize both reported isoforms (NP_001025444.1 and NP_001025443.1). There are reported variants which represent identical protein (NP_001025443.1 and NP_001169.3).
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Horse, Chicken, Hamster, Orangutan
Immunogen
Synthetic peptide: REKITTNCYKFKIKD, corresponding to internal sequence amino acids 398 - 410 of Human BMAL1 (NP_001169.3 and NP_001025444.1)
REKITTNCYK FKIKD
Positive control
Human Brain (Cerebellum) lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab81974 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Blood >> Blood Pressure regulation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Neuroscience >> Neurology process >> Metabolism
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
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Western blot - BMAL1 antibody (ab81974)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: antibody detection limit dilution 1/8000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 69 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.
Tissue specificity
Highly expressed in the adult brain, skeletal muscle and heart.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression.
Phosphorylated upon dimerization with CLOCK.
Sumoylated on Lys-259 upon dimerization with CLOCK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO00327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARNT like protein 1 brain and muscle antibody
- Arntl antibody
- Aryl hydrocarbon receptor nuclear translocator like antibody
see all
Database links
- Entrez Gene: 406 Human
- Entrez Gene: 11865 Mouse
- Entrez Gene: 29657 Rat
- Omim: 602550 Human
- SwissProt: O00327 Human
- SwissProt: Q9WTL8 Mouse
- SwissProt: Q9EPW1 Rat
- Unigene: 65734 Human
see all
Anti-BMAL1 antibody images:
Western blot - BMAL1 antibody (ab81974)
Anti-BMAL1 antibody (ab81974) at 0.3 µg/ml + Human Brain Cerebellum lysate (in RIPA buffer) at 35 µg/ml
Predicted band size : 69 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-BMAL1 antibody (ab81974)
ab81974 has not yet been referenced specifically in any publications.
Publishing research using ab81974? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"