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Products:Neuroscience >> Neurology process >> Circadian Rhythm
Overview
Product name
Anti-KAT13D / CLOCK antibody
KAT13D / CLOCK 抗体 (9件) 一覧
Description
Rabbit polyclonal to KAT13D / CLOCK
Specificity
ab65033 detects endogenous levels of total KAT13D / CLOCK protein.
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from an internal sequence of human KAT13D / CLOCK
Positive control
IHC-P: Human skeletal muscle tissue. WB: HUVEC cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab65033 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Blood >> Blood Pressure regulation
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Neurology process >> Circadian Rhythm
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Target
Function
ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
Tissue specificity
Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.
Cellular localization
Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.
Target information above from: UniProt accessionO15516
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHe8 antibody
- Circadian locomoter output cycles kaput protein antibody
- Circadian locomoter output cycles protein kaput antibody
see all
Database links
- Entrez Gene: 9575 Human
- Entrez Gene: 12753 Mouse
- Entrez Gene: 60447 Rat
- Omim: 601851 Human
- SwissProt: O15516 Human
- SwissProt: O08785 Mouse
- SwissProt: Q9WVS9 Rat
- Unigene: 436975 Human
see all
Anti-KAT13D / CLOCK antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAT13D / CLOCK antibody (ab65033)
ab65033, at a 1/50 dilution, staining KAT13D / CLOCK in paraffin embedded human skeletal muscle tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
Western blot - KAT13D / CLOCK antibody (ab65033)
All lanes : Anti-KAT13D / CLOCK antibody (ab65033) at 1/500 dilution
Lane 1 : HUVEC cell extract
Lane 2 : HUVEC cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 95 kDa
Observed band size : 95 kDa
References for Anti-KAT13D / CLOCK antibody (ab65033)
This product has been referenced in:
- Nagalla Set al. Platelet microRNA-mRNA coexpression profiles correlate with platelet reactivity. Blood 117:5189-97 (2011). WB; Human.Read more (PubMed: 21415270) »
See 1 publication for this product
Publishing research using ab65033? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"