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Products:Neuroscience >> Neurology process >> Circadian Rhythm
Overview
Product name
Anti-Cryptochrome I antibody
Cryptochrome I 抗体 (3件) 一覧
Description
Mouse monoclonal to Cryptochrome I
Tested applications
WB, ICC/IF, IHC-P, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-587 of Human Cryptochrome I
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Neuroscience >> Sensory System >> Visual system
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Neuroscience >> Neurology process >> Circadian Rhythm >> Receptors
Neuroscience >> Neurology process >> Circadian Rhythm
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 66 kDa.
ICC/IF: Use a concentration of 10 µg/ml
IHC-P: Use a concentration of 5 µg/ml
Flow Cyt: Use 1µg for 106 cells.
Target
Function
Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK
NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK
NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.
Sequence similarities
Belongs to the DNA photolyase class-1 family.
Contains 1 DNA photolyase domain.
Post-translational
modifications
Phosphorylation on Ser-247 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSNK1E requires interaction with PER1 or PER2.
Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex leading to degradation.
Cellular localization
Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.
Target information above from: UniProt accessionQ16526
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- PHLL1 antibody
- CRY1 antibody
- CRY1_HUMAN antibody
see all
Database links
- Entrez Gene: 1407 Human
- Omim: 601933 Human
- SwissProt: Q16526 Human
- Unigene: 151573 Human
Anti-Cryptochrome I antibody images:
Immunofluorescence - Cryptochrome I antibody (ab54649)
Cryptochrome I antibody (ab54649) used in immunofluorescence at 10ug/ml on HeLa cells.
IHC-P - Cryptochrome I antibody (ab54649)
Cryptochrome I antibody (ab54649) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon adenocarcinoma tissue.
Western blot - Anti-Cryptochrome I antibody (ab54649)
Predicted band size : 66 kDa
Cryptochrome I antibody (ab54649) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry-Cryptochrome I antibody(ab54649)
Overlay histogram showing HeLa cells stained with ab54649 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54649, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-Cryptochrome I antibody (ab54649)
ab54649 has not yet been referenced specifically in any publications.
Publishing research using ab54649? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"