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Products:Neuroscience >> Neurology process >> Circadian Rhythm
Overview
Product name
Anti-KAT13D / CLOCK antibody [648C1a]
KAT13D / CLOCK 抗体 (9件) 一覧
Description
Mouse monoclonal [648C1a] to KAT13D / CLOCK
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human CLOCK fragment
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, 8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
648C1a
Isotype
IgG1
Research areas
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Blood >> Blood Pressure regulation
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Neurology process >> Circadian Rhythm
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 95 kDa.
Dot: Use at an assay dependent dilution.
Target
Function
ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
Tissue specificity
Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.
Cellular localization
Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.
Target information above from: UniProt accessionO15516
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHe8 antibody
- Circadian locomoter output cycles kaput protein antibody
- Circadian locomoter output cycles protein kaput antibody
see all
Database links
- Entrez Gene: 9575 Human
- Omim: 601851 Human
- SwissProt: O15516 Human
- Unigene: 436975 Human
References for Anti-KAT13D / CLOCK antibody [648C1a] (ab51333)
ab51333 has not yet been referenced specifically in any publications.
Publishing research using ab51333? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"