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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-BMAL1 antibody
BMAL1 抗体 (8件) 一覧
Description
Rabbit polyclonal to BMAL1
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Cow, Dog, Pig
Immunogen
A region within synthetic peptide: RKRKGSATDY QESMDTDKDD PHGRLEYAEH QGRIKNAREA HSQIEKRRRD, corresponding to amino acids 37-86 of Mouse BMAL1
RKRKGSATDY QESMDTDKDD PHGRLEYAEH QGRIKNAREA HSQIEKRRRD
Positive control
NIH3T3 lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Blood >> Blood Pressure regulation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Neuroscience >> Neurology process >> Metabolism
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
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Western blot - BMAL1 antibody (ab49421)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Target
Function
ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.
Tissue specificity
Highly expressed in the adult brain, skeletal muscle and heart.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression.
Phosphorylated upon dimerization with CLOCK.
Sumoylated on Lys-259 upon dimerization with CLOCK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO00327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARNT like protein 1 brain and muscle antibody
- ARNTL antibody
- Aryl hydrocarbon receptor nuclear translocator like antibody
see all
Database links
- Entrez Gene: 476860 Dog
- Entrez Gene: 11865 Mouse
- Entrez Gene: 29657 Rat
- SwissProt: Q9WTL8 Mouse
- SwissProt: Q9EPW1 Rat
- Unigene: 440371 Mouse
- Unigene: 14532 Rat
Anti-BMAL1 antibody images:
Western blot - BMAL1 antibody (ab49421)
Anti-BMAL1 antibody (ab49421) at 1.25 µg/ml + NIH3T3 lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 -1/100,000 dilution
Predicted band size : 26 kDa
Observed band size : 26 kDa
References for Anti-BMAL1 antibody (ab49421)
This product has been referenced in:
- Cherrington BDet al. NeuroD1 and Mash1 temporally regulate GnRH receptor gene expression in immortalized mouse gonadotrope cells. Mol Cell Endocrinol 295:106-14 (2008).Read more (PubMed: 18760324) »
See 1 publication for this product
Publishing research using ab49421? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"