製品の概要

  • 製品名Anti-INCENP antibody
    INCENP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to INCENP
  • アプリケーション適用あり: WB, ICC/IF, ICC, IHC-Fr, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Xenopus laevis
    交差が予測される動物種: Chicken
  • 免疫原

    Synthetic peptide:

    SKPRYHKRTSSAVWNSP

    (with N-terminal added cysteine) conjugated to KLH, corresponding to amino acids 884-901 of Human INCENP.

  • ポジティブ・コントロール
    • Nuclear/whole extract of human HeLa cells.
  • 特記事項If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells (see image below). However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
ICC/IF Use at an assay dependent concentration.
ICC 1/100.
IHC-Fr Use at an assay dependent concentration.
IP 1/250.

ターゲット情報

  • 機能Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.
  • 配列類似性Belongs to the INCENP family.
  • 細胞内局在Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • binds and activates aurora B and C in vivo and in vitro antibody
    • Chromosomal passenger protein antibody
    • INCE_HUMAN antibody
    • INCENP antibody
    • Inner centromere protein antibody
    • Inner centromere protein antigens 135/155kDa antibody
    • Inner centromere protein antigens 135kD 155kD antibody
    • Inner centromere protein INCENP antibody
    see all

Anti-INCENP antibody 画像

  • HeLa cells were fixed for 10mins in fresh 3.7% paraformaldehyde, permeabilised with PBS + 0.1% Triton X 100, and blocked with 1% goat serum in Abdil. The primary antibody (in Abdil) was incubated OVERNIGHT (1 hour didn't work) at room temperature at a dilution of 1/150. The cells were co-stained with mouse anti-tubulin (secondary is Anti-Mouse Alexa 488) and ACA (Human anti centromere marker, secondary is Anti-Human Cy5) and DAPI. The figure shows projected images of dividing cells (prophase, prometaphase, metaphase, anaphase, telophase). The top panels show ab12183, DNA and microtubule stains. The lower panels are the same cells, showing a subset of optical sections at higher magnification. ACA (geen) stains centromeres and ab12183 (red) stains the inner centromere in prophase, prometaphase, and metaphase. The ab12183 channel has been linerally scaled to reduce the background staining associated with this antibody.
  • Demembranated sperm nuclei and CSF (cytostatic factor-arrested) low-speed egg extracts were prepared in XB and reacted at room temperature as described. Proteins were added to extract prior to sperm addition or else immediately after sperm addition into XB buffer (100 mM KCl, 1 mM MgCl2, 0.1 mM CaCl2, 10 mM K-HEPES pH 7.7, 50 mM sucrose) supplemented with energy mix (3.75 mM creatinine phosphate, 0.5 mM Na2-ATP, 0.5 mM MgCl2, 50 µM EGTA). To label damaged DNA, biotin-16-dUTP was added to metaphase reactions to a final concentration of 40 µM. 30 minutes after sperm addition, reactions were diluted 1/10 into XB supplemented with 1 mM MgCl2, 5 mM EGTA, 0.25% Triton X-100, and 10% formaldehyde and processed for Immunofluorescence. Antibody used ab12183 (red).
  • Immunohistochemistry (Frozen sections) analysis of mouse embryonic skin - keratinocytes tissue sections labeling INCENP with ab12183. Tissue was fixed with paraformaldehyde, permeabilized with 10% Triton-X (0.05% Final) and blocked with Gelatin for 1 hour at 20°C. Samples were incubated with primary antibody (1/200) for 12 hours at 4°C. An undiluted Alexa Fluor 488 donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-INCENP antibody (ab12183) 使用論文

This product has been referenced in:
  • Hebras C & McDougall A Urochordate Ascidians Possess a Single Isoform of Aurora Kinase That Localizes to the Midbody via TPX2 in Eggs and Cleavage Stage Embryos. PLoS One 7:e45431 (2012). ICC/IF . Read more (PubMed: 23029005) »
  • Schoenmakers S  et al. Meiotic silencing and fragmentation of the male germline restricted chromosome in zebra finch. Chromosoma 119:311-24 (2010). ICC/IF ; Zebra finch . Read more (PubMed: 20162291) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic Skin - keratinocytes in vivo)
Permeabilization Yes - 10% Triton-X (0.05% Final)
Specification Embryonic Skin - keratinocytes in vivo
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Dr. Scott Williams

Verified customer

投稿 Sep 30 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample Human Cell (293T)
Specification 293T
Permeabilization Yes - 0.5% Triton X100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 21 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon carcinoma)
Specification Colon carcinoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Dec 03 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Methanol
Permeabilization Yes - 0.1% triton X-100
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% in
Username

Dr. William Moore

Verified customer

投稿 Nov 30 2005

Application Immunocytochemistry
Sample Human Cell (HeLa and SK-N-SH)
Specification HeLa and SK-N-SH
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Oct 12 2005

I have received confirmation from the source of ab12183 that the antibody will detect a 106 kDa band using whole HeLa extract. They cannot explain why you are detecting a 80kDa band and hypothesize that this could be a splice variant or a lysed form of...

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We have just received the following information from the source of ab12183 who tested the antibody in HeLa nuclear extracts: 1. Extract: 10^7 cells/slab of HeLa nuclear. 2.Wet transfer for 90 minutes at 4'C. 3. Blocking solution: 5%-10% non fat dr...

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Thanks for your email. I can send you ab2270 as a replacement. There's no need to return ab12183. Can you please confirm your shipping address, including phone number?

Thank you for your enquiry, and I'm sorry to hear that you are experiencing difficulty with ab12183. I would like to make some suggestions in order to assist you. First, I suggest running a positive control. For this antibody nuclear/whole extract of h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"