In situ Direct DNA Fragmentation (TUNEL) Assay Kit (ab66108)


  • 製品名In situ Direct DNA Fragmentation (TUNEL) Assay Kit
    DNA fragments キット 製品一覧
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプDirect
  • 製品の概要

    Abcam's In situ Direct DNA Fragmentation (TUNEL) Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3'-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be analyzed by flow cytometry.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    This kit is FITC-labeled (Ex/Em = 495/519nm). If want to use BrdU-Red (Ex/Em = 488/576nm) as a label, we recommend our In situ BrdU-Red DNA Fragmentation (TUNEL) Assay Kit (ab66110).

  • アプリケーション適用あり: Flow Cytmore details


  • 関連性Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.


Our Abpromise guarantee covers the use of ab66108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

In situ Direct DNA Fragmentation (TUNEL) Assay Kit 画像

  • Control RAW 264.7 cells.

  • RAW 264.7 cells treated with 2 μM camptothecin (ab120115) for 24 hours prior to staining.

  • RAW 264.7 cells treated with 10 μM camptothecin (ab120115) for 24 hours prior to staining.


In situ Direct DNA Fragmentation (TUNEL) Assay Kit (ab66108) 使用論文

This product has been referenced in:
  • Chen J  et al. Antithrombin nanoparticles improve kidney reperfusion and protect kidney function after ischemia-reperfusion injury. Am J Physiol Renal Physiol 308:F765-73 (2015). Read more (PubMed: 25651565) »

See 1 Publication for this product

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The laboratory responded to your inquiry regarding visibility of a pellet after adding the washing buffer. You should be able to see at least a mini-speck of the pellet even after the wash step. One easy thing to do would be to take an aliquot of ...

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We have not tried this protocol on tissue sections, but here is a protocol that the lab has found from another which can be modified to fit into this procedure. A. Tissue Section Preparations: The protocol describes the preparation of formali...

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Thank you for your inquiry and your patience while we looked into this further.

For ab66108, the kit components are optimized for usage on cells. If using tissue sections, you will not be able to get a full 50 assays from the kit because you ...

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Thank you for contacting us. This kit will work fine with the samples you describe.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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I discussed with my colleagues from the Scientific Support Team about the use of these kits in yeast and we agreed that the kit that is likely to work is the In situ Direct DNA Fragmentation Assay Kit (ab66108) but no...

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Thank you for your enquiry and your interest in our products. The two kits (ab66108 and ab66110) are mainly used for cells analyzed by flow cytometry. Currently, we do not have specific kits for tissue samples (for DNA Fragmentation) but I would...

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