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Abcam's In situ BrdU-Red DNA Fragmentation (TUNEL) Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a Red fluorescence labeled anti-BrdU monoclonal antibody. The assay is suitable for studying apoptosis with GFP transfected cells.
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This kit is BrdU-Red labeled (Ex/Em = 488/576nm). If want to use FITC (Ex/Em = 495/519nm) as a label, we recommend our In situ Direct DNA Fragmentation (TUNEL) Assay Kit (ab66108).
|7-AAD/RNase Staining Buffer||Amber bottle||1 x 30ml|
|Anti-BrdU-Red Antibody||Orange||1 x 300µl|
|Br-dUTP||Violet||1 x 480µl|
|Negative Control Cells||Neutral||1 x 5ml|
|Positive Control Cells||Brown||1 x 5ml|
|Reaction Buffer||Green||1 x 600µl|
|Rinse Buffer||Red||1 x 120ml|
|TdT Enzymes||1 x 45µl|
|Wash Buffer||Blue||1 x 120ml|
Our Abpromise guarantee covers the use of ab66110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
TUNEL staining in whole mount Hydractinia echinata using In situ BrdU-Red DNA Fragmentation (TUNEL) Assay Kit (ab66110).
Animals were fixed in 4% PFA in PBS for 1 hour and processed as per the protocol without proteinaseK treatment. In place of proteinaseK animals were permeabilised in 3% Triton in PBS for 15 minutes. Animals were counter-stained with DAPI.