In-Cell ELISA (ICE) Support Pack w/o plates (ab111541)

製品の概要

  • 製品名In-Cell ELISA (ICE) Support Pack w/o plates
    In cell elisa キット 製品一覧
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプCell-based (quantitative)
  • ステップMultiple steps standard assay
  • 製品の概要

    Note - This kit does not include cell culture plates. 96 well or 384 well plates which are appropriate for the requirements of your cell line must be purchased seperately. However an alternative support pack, ab111542 which contains five high quality 96-well microplates suitable for ICE is available.


    ab111541 is for use with suspension, apoptotic/detaching cells and adherent cell lines. The pack contains buffers and a protocol to perform ICE with Abcam ICE-validated antibodies. For an ICE assay, it is necessary to purchase both primary antibody(ies) and labeled secondary antibody(ies). Antibodies are sold separately, allowing customizing the target(s) of interest, method of detection and multiplexing.


    In-Cell ELISA uses quantitative immunocytochemistry to measure protein levels or post-translational modifications within cells. The cells are fixed to the bottom of a coated 96-well plate (not provided). Targets of interest are detected by primary antibodies, which are in turn quantified with labeled secondary antibody(ies). Abcam offers highly-specific, well-characterized primary antibodies, IRDye®- or HRP-labeled anti-mouse and anti-rabbit secondary antibodies, as well as IRDye®-labeled isotype specific anti-mouse antibodies. By combining antibodies of different species or isotype and appropriate IR-labeled secondary antibodies, two color multiplexing can be achieved in the 800/700 channels. IR imaging and quantification is performed using a LI-COR® Odyssey® or Aerius® system. HRP-labeled complexes are developed and quantified colorimerically using spectrophotometer.


    Two protocols are available when using this kit. Protocol (1) suspension cells and cells likely to detach under experimental conditions (for example, adherent cells undergoing apoptosis readily detach from a culture plate). Protocol (2) - A second protocol is available for normal adherent cells. These protocols can be used with any of Abcam's ICE-validated antibodies. Specific scientific information, background and working concentration for each antibody are detailed in each antibody's corresponding datasheet.

  • アプリケーション適用あり: In-Cell ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 保存方法Store at +4°C. Please refer to protocols.
  • 内容 5 x 96 tests
    100X Triton X-100 1 x 1.25ml
    10X Blocking Solution 1 x 40ml
    10X Phosphate Buffered Saline (PBS) 1 x 250ml
    400X Tween-20 1 x 4ml
    Janus Green Stain 1 x 30ml

    アプリケーション

    Our Abpromise guarantee covers the use of ab111541 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    In-Cell ELISA Use at an assay dependent dilution.

    In-Cell ELISA (ICE) Support Pack w/o plates 画像

    • Cells are grown to ~80% confluency in a 96- or 384-well plate, a drug/other treatment is applied to stimulate a cellular response. The cells are then fixed and permeabilized, effectively "freezing" them. Primary antibodies are then added which bind to their intended targets within the mitochondria or other subcellular compartment. After incubation, the unbound primary antibodies are washed away and secondary antibodies are added. These secondaries are conjugated to either IRDyes® or to an enzyme label (HRP or AP) for the colorimetric versions of the assays. Unbound secondaries are washed away, reaction buffer is added for the colorimetric assays, and the signal is read on a suitable instrument for the kit type.
      » In-cell ELISA diagram in PDF format

    プロトコール

    In-Cell ELISA (ICE) Support Pack w/o plates (ab111541) 使用論文

    ab111541 has not yet been referenced specifically in any publications.

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