製品の概要
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製品名Anti-Importin4 antibody
Importin4 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Importin4
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由来種Rabbit
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アプリケーション適用あり: IP, IHC-Pmore details
適用なし: WB -
種交差性交差種: Human
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免疫原
Synthetic peptide corresponding to a region within amino acids 900-950 (LLPVLLSTAQ EADPEVRSNA IFGMGVLAEH GGHPAQEHFP KLLGLLFPLL A) of Human Importin4 (NP_078934.3).
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ポジティブ･コントロール
- HeLa whole cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab99271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at 5-15 µg/mg of lysate. | |
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
追加情報
Is unsuitable for WB.
ターゲット情報
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機能Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
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配列類似性Belongs to the importin beta family.
Contains 6 HEAT repeats.
Contains 1 importin N-terminal domain. -
細胞内局在Cytoplasm. Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 79711 Human
- SwissProt: Q8TEX9 Human
- Unigene: 411865 Human
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別名
- Imp4 antibody
- Imp4b antibody
- Importin 4 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4 antibody (ab99271)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Importin4 with ab99271 at 1/5000 (0.2µg/mg). Detection: DAB. -
Immunoprecipitation - Importin4 antibody (ab99271)Detection of Importin4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99271 at 10 µg/mg lysate for IP. ab99270, which recognizes an upstream epitope, was used at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 5 seconds.
参考文献
This product has been referenced in:
- Wei Y et al. Importin 8 regulates the transport of mature microRNAs into the cell nucleus. J Biol Chem 289:10270-5 (2014). IP ; Mouse . Read more (PubMed: 24596094) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"