製品の概要

  • 製品名Anti-Importin4 antibody
    Importin4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Importin4
  • アプリケーション適用あり: IP, IHC-Pmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 900-950 (LLPVLLSTAQ EADPEVRSNA IFGMGVLAEH GGHPAQEHFP KLLGLLFPLL A) of Human Importin4 (NP_078934.3).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
    • 配列類似性Belongs to the importin beta family.
      Contains 6 HEAT repeats.
      Contains 1 importin N-terminal domain.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Imp4 antibody
      • Imp4b antibody
      • Importin 4 antibody
      • Importin 4b antibody
      • Importin-4 antibody
      • Importin-4b antibody
      • Ipo4 antibody
      • IPO4_HUMAN antibody
      • Ran binding protein 4 antibody
      • Ran-binding protein 4 antibody
      • RanBP4 antibody
      see all

    Anti-Importin4 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Importin4 with ab99271 at 1/5000 (0.2µg/mg). Detection: DAB.
    • Detection of Importin4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99271 at 10 µg/mg lysate for IP. ab99270, which recognizes an upstream epitope, was used at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 5 seconds.

    Anti-Importin4 antibody (ab99271) 使用論文

    This product has been referenced in:
    • Wei Y  et al. Importin 8 regulates the transport of mature microRNAs into the cell nucleus. J Biol Chem 289:10270-5 (2014). IP ; Mouse . Read more (PubMed: 24596094) »

    See 1 Publication for this product

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