製品の概要

  • 製品名Anti-Importin4 antibody
    Importin4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Importin4
  • アプリケーション適用あり: IP, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 350-400 (CPQLMPMLEE ALRSESPYQR KAGLLVLAVL SDGAGDHIRQ RLLPPLLQIV C) of Human Importin4 (NP_078934.3).

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa. This antibody has not performed satisfactorily when used for WB of Importin4 in crude preparations (e.g. whole cell lysate). ab99270 can be used for WB of enriched (e.g. immunoprecipitated) sources of Importin4.

ターゲット情報

  • 機能Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • 配列類似性Belongs to the importin beta family.
    Contains 6 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Imp4 antibody
    • Imp4b antibody
    • Importin 4 antibody
    • Importin 4b antibody
    • Importin-4 antibody
    • Importin-4b antibody
    • Ipo4 antibody
    • IPO4_HUMAN antibody
    • Ran binding protein 4 antibody
    • Ran-binding protein 4 antibody
    • RanBP4 antibody
    see all

Anti-Importin4 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Importin4 with ab99270 at 1/5000 (0.2µg/mg). Detection: DAB.
  • Detection of Importin4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99270 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 5 seconds

Anti-Importin4 antibody (ab99270) 使用論文

ab99270 has not yet been referenced specifically in any publications.

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