Anti-Importin 9/RANBP9 抗体 [EPR1352] (ab124710)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1352] to Importin 9/RANBP9
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Importin 9/RANBP9 antibody [EPR1352]
Importin 9/RANBP9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1352] to Importin 9/RANBP9 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa, SKBR-3, C6, and NIH/3T3 cell lysates and Human brain tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 2.29 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1352 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 116 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6. -
配列類似性
Belongs to the importin beta family.
Contains 1 importin N-terminal domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 55705 Human
- Entrez Gene: 226432 Mouse
- Entrez Gene: 304817 Rat
- SwissProt: Q96P70 Human
- SwissProt: Q91YE6 Mouse
- Unigene: 596014 Human
- Unigene: 271642 Mouse
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別名
- Imp9 antibody
- Imp9a antibody
- Imp9b antibody
see all
画像
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All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IPO9 knockout HeLa cell lysate
Lane 3 : SK-BR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 116 kDaLanes 1-3: Merged signal (red and green). Green - ab124710. Red - loading control, ab8245 observed at 36 kDa.
ab124710 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265352 (knockout cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab124710 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 116 kDa -
ab124710, at 1/500 to 1/1000, staining Impotin 9/RANBP9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab124710 は 2 報の論文で使用されています。
- Hirai Y et al. Intracellular dynamics of actin affects Borna disease virus replication in the nucleus. Virus Res 263:179-183 (2019). PubMed: 30769121
- Liu C et al. Nuclear Actin Polymerized by mDia2 Confines Centromere Movement during CENP-A Loading. iScience 9:314-327 (2018). PubMed: 30448731