製品の概要

  • 製品名Anti-Importin 8 antibody
    Importin 8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Importin 8
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human, Chimpanzee, Monkey
    交差が予測される動物種: Mouse, Chicken, Cow, Dog
  • 免疫原

    A portion of amino acids 850-900 of human Importin 8

  • ポジティブ・コントロール
    • HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
ICC/IF Use a concentration of 5 - 10 µg/ml.

ターゲット情報

  • 機能Seems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19.
  • 配列類似性Belongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ26580 antibody
    • Imp 8 antibody
    • Imp8 antibody
    • Importin-8 antibody
    • Importin8 antibody
    • IPO 8 antibody
    • IPO8 antibody
    • IPO8_HUMAN antibody
    • Ran binding protein 8 antibody
    • RAN BP8 antibody
    • Ran-binding protein 8 antibody
    • RANBP 8 antibody
    • RanBP8 antibody
    see all

Anti-Importin 8 antibody 画像

  • All lanes : Anti-Importin 8 antibody (ab72109) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with immunizing peptide


    Predicted band size : 120 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • Staining of HeLa cells using ab72109 (left) or a control rabbit Ig (right) at 5ug/ml.

Anti-Importin 8 antibody (ab72109) 使用論文

ab72109 has not yet been referenced specifically in any publications.

Product Wall

Thank you for this link. So as I understand it, our target for this product is Importin 8, which is different from Importin alpha 8. Importin alpha 8 has an alternative name of KPNA7 and is ˜ 56 kD, while importin 8 iteself is a larger protein ˜ 120 kD...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"